产品名称
MEM 非必需氨基酸溶液 (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
General description
Eagle′s基本必需培养基(MEM)是一种合成培养基，由平衡盐溶液、氨基酸和维生素组成。这些组份为培养细胞生长的必需组份。MEM可添加非必需氨基酸，而制备MEM非必需氨基酸溶液。非必需氨基酸包括L-丙氨酸、L-天冬酰胺、L-天冬氨酸、L-甘氨酸、L-丝氨酸、L-脯氨酸和L-谷氨酸。
Application
向培养基中添加氨基酸补充剂不仅可以刺激细胞生长还可以延长培养细胞的活力。MEM非必需氨基酸溶液的补充物，可增加营养成分并减少 体外细胞的生物合成负担。
MEM非必需氨基酸溶液已可用于维持哺乳动物细胞的Dulbecco′s基本必需培养基。它已被添加到卵母细胞成熟培养基中。
MEM非必需氨基酸溶液已可用于维持哺乳动物细胞的Dulbecco′s基本必需培养基。它已被添加到卵母细胞成熟培养基中。
Biochem/physiol Actions
杂交瘤培养研究表明，Eagle8242;s基本培养基(MEM)添加非必需氨基酸可提高杂交瘤细胞在死亡阶段的膜稳定性。这也会导致死亡阶段抗体分泌增加，乳酸浓度下降。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Ayako Suzuki et al.
Genome biology, 16, 66-66 (2015-04-19)
To understand the heterogeneous behaviors of individual cancer cells, it is essential to investigate gene expression levels as well as their divergence between different individual cells. Recent advances in next-generation sequencing-related technologies have enabled us to conduct a single-cell RNA-Seq
Cell Culture
Jakoby WB
Methods in Enzymology, 58 (1979)
Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures
Fassnacht D
Biotechnology Letters, 19(1), 35-38 (1997)
Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents.
Hashimoto S et al.
Molecular Reproduction and Development, 56, 520-520 (2000)
Jeffrey E McLean et al.
The Journal of biological chemistry, 286(25), 22147-22159 (2011-04-23)
Flaviviruses include the most prevalent and medically challenging viruses. Persistent infection with flaviviruses of epithelial cells and hepatocytes that do not undergo cell death is common. Here, we report that, in epithelial cells, up-regulation of autophagy following flavivirus infection markedly
商品
Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.
实验方案
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M7145-100ML | 04061838259813 |
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