Monoclonal Anti-MEKK4 antibody produced in mouse

Monoclonal Anti-MEKK4 (mouse IgG2b isotype) is derived from the hybridoma MEKK4-338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MEKK4. MAP kinase 4 (MEKK4 also called MAP3K4, is the human homolog of the S. cerevisiae Ssk2 and Ssk22 MAPKKKs. The protein has 1607 amino acids with a protein kinase catalytic domain at the C-terminus that is 98, 42, 36 and 41 percent identical to the kinase domains of mouse MEKK4, Serine/threonine-protein kinase proteins (SSK22 - Ssk2, Ssk22) and S. pombe Wik1, respectively. A non-kinase domain that has a regulatory function is located at the N-terminal part of the protein. The protein is expressed mainly in heart, placenta, skeletal muscle and pancreas.

synthetic peptide corresponding to amino acids 1081-1095 of human MEKK4, conjugated to KLH.

Monoclonal Anti-MEKK4 antibody produced in mouse has been used in:

• western blotting
• ELISA
• immunocytochemistry

MAP kinase 4 (MEKK4) -null embryos developed highly penetrant neural tube defects with exencephaly and spina bifida. During neural tube development, it regulates MAP kinase 4 (MKK4) activity and apoptotic cell death.

Monoclonal Anti-MEKK4 antibody is specific for human, rat and mouse MEKK4 (approx. 182 kDa).

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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