biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~40 kDa
species reactivity
human, mouse (predicted), rat (predicted)
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~2 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using HEK-293T cells expressing human MO25 and PC12 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CAB39(51719)
mouse ... Cab39(12283)
rat ... Cab39(301574)
General description
MO25 is a 40 kDa scaffolding protein that interacts with STRADα and LKB1 to regulate tumor suppression. MO25 also facilitates the activation and cytoplasmic localization of LKB1 . Anti-MO25 (C-terminus) antibody detects human MO25 (approx. 40 kDa). The product is also predicted to react with rat and mouse MO25. Staining of the MO25 band in immunoblotting is specifically inhibited by the immunizing peptide.
Immunogen
synthetic peptide corresponding to amino acids 323-341 located near the C-terminus of human MO25. This sequence is identical in chicken, dog, and bovine MO25 and highly conserved in rat and mouse MO25 (2 amino acid substitutions).
Application
Anti-MO25 (C-terminus) antibody is suitable for use in western blot (0.5-1 μg/mL using HEK-293T cells expressing human MO25 and in PC12 cell lysates).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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相关内容
Instructions
Elton Zeqiraj et al.
Science (New York, N.Y.), 326(5960), 1707-1711 (2009-11-07)
The LKB1 tumor suppressor is a protein kinase that controls the activity of adenosine monophosphate-activated protein kinase (AMPK). LKB1 activity is regulated by the pseudokinase STRADalpha and the scaffolding protein MO25alpha through an unknown, phosphorylation-independent, mechanism. We describe the structure
Jérôme Boudeau et al.
The EMBO journal, 22(19), 5102-5114 (2003-10-01)
Mutations in the LKB1 protein kinase result in the inherited Peutz Jeghers cancer syndrome. LKB1 has been implicated in regulating cell proliferation and polarity although little is known about how this enzyme is regulated. We recently showed that LKB1 is
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M7195-200UL | 04061838173287 |
| M7195-25UL | 04061838031822 |