M7292
M16 培养基
With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
别名:
M16培养基溶液, 细胞培养基, 细胞培养基M16
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | embryo: suitable
杂质
endotoxin, tested
组分
NaHCO3: 2.101 g/L
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L
glucose: 1.0 g/L (Dextro)
运输
ambient
储存温度
2-8°C
应用
M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的 Krebs-Ringer 碳酸氢盐溶液，与 Whitten′s 培养基非常相似。M16 含有丙酮酸和乳酸作为能量来源，因为植入前胚胎不能有效利用葡萄糖。
制备说明
添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。
分析说明
溶液中的沉淀不影响本品性能。
该产品经过测试，能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80％。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Qian-Qian Sha et al.
Nature communications, 9(1), 3477-3477 (2018-08-30)
Trimethylation of histone H3 on lysine-4 (H3K4me3) is associated with gene-regulatory elements, but its transcription-independent function in cell division is unclear. CxxC-finger protein-1 (CFP1) is a major mediator of H3K4 trimethylation in mouse oocytes. Here we report that oocyte-specific knockout
Hong-Bin Liu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 6(15), 1900295-1900295 (2019-08-14)
A number of genes involved in zygotic genome activation (ZGA) have been identified, but the RNA-binding maternal factors that are directly related to ZGA in mice remain unclear. The present study shows that maternal deletion of Igf 2bp2 (also commonly known
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
Marie-Christine Birling et al.
Scientific reports, 7, 43331-43331 (2017-03-08)
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of
Xinyan Cao et al.
International journal of molecular sciences, 20(9) (2019-05-01)
Embryo implantation in the mink follows the pattern of many carnivores, in that preimplantation embryo diapause occurs in every gestation. Details of the gene expression and regulatory networks that terminate embryo diapause remain poorly understood. Illumina RNA-Seq was used to
商品
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂
实验方案
使用CRISPR/Cas9系统生成正确的转基因小鼠需要充分理解正在测试的假设。了解如何创建成功的小鼠模型项目。
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