Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)
shipped in
ambient
storage temp.
2-8°C
General description
MCDB 153培养基是对Ham营养混合物F-12的改良,设计用于人角质形成细胞的生长、鸡胚成纤维细胞的克隆生长和中国仓鼠卵巢(CHO)细胞的生长,使用低水平的胎牛血清蛋白(FBSP)、大量微量元素或无血清蛋白。
Application
MCDB 153培养基已用作黑色素瘤分离培养基(MIM)的组分,用于培养人类非转移性和转移性黑色素瘤细胞。
Preparation Note
并补充1.18 g/L碳酸氢钠。
配方中每升培养基含有17.6克的粉末。
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable