M7403
MCDB 153培养基
With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
别名:
Molecular, Cellular, and Development Biology 153 medium
产品名称
MCDB 153培养基, With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)
shipped in
ambient
storage temp.
2-8°C
Quality Level
Application
MCDB 153培养基已用作黑色素瘤分离培养基（MIM）的组分，用于培养人类非转移性和转移性黑色素瘤细胞。
General description
MCDB 153培养基是对Ham营养混合物F-12的改良，设计用于人角质形成细胞的生长、鸡胚成纤维细胞的克隆生长和中国仓鼠卵巢（CHO）细胞的生长，使用低水平的胎牛血清蛋白（FBSP）、大量微量元素或无血清蛋白。
Preparation Note
并补充1.18 g/L碳酸氢钠。
配方中每升培养基含有17.6克的粉末。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Hans Peter Weitzenböck et al.
Cancer medicine, 11(4), 956-967 (2021-12-25)
Malignant melanoma is the deadliest form of skin cancer and NRF2 has been proposed as a main regulator of tumor cell malignancy. Still the mechanisms how NRF2 is contributing to melanoma progression are incompletely understood. Here we analyzed the effects
Andrew S McNeal et al.
eLife, 10 (2021-11-24)
Benign melanocytic nevi frequently emerge when an acquired BRAFV600E mutation triggers unchecked proliferation and subsequent arrest in melanocytes. Recent observations have challenged the role of oncogene-induced senescence in melanocytic nevus formation, necessitating investigations into alternative mechanisms for the establishment and
Yochai Wolf et al.
Cell, 179(1), 219-235 (2019-09-17)
Although clonal neo-antigen burden is associated with improved response to immune therapy, the functional basis for this remains unclear. Here we study this question in a novel controlled mouse melanoma model that enables us to explore the effects of intra-tumor
Matthew L Fisher et al.
Molecular carcinogenesis, 55(12), 2024-2036 (2015-12-24)
Melanoma is a metastatic cancer associated with poor survival. Here, we study a subpopulation of melanoma cancer cells displaying melanoma cancer stem cell (MCS cells) properties including elevated expression of stem cell markers, increased ability to survive as spheroids, and
Dominik Brokatzky et al.
The EMBO journal, 38(11) (2019-04-14)
Apoptosis is a frequent form of programmed cell death, but the apoptotic signaling pathway can also be engaged at a low level, in the absence of cell death. We here report that such sub-lethal engagement of mitochondrial apoptosis signaling causes
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
相关内容
Instructions
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