M7528
培养基 199
HEPES modification, with Earle′s salts, and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
细胞培养基
Product Name
培养基 199, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
pH值（酸碱度）
>7.2
组分
L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes
运输
ambient
储存温度
2-8°C
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一般描述
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
制备说明
补充 0.1 g/L L -谷氨酰胺。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Marten Michaelis et al.
Andrology, 8(5), 1419-1427 (2020-04-20)
Recently we described two outbred mouse lines which have been selected for high fertility. These mouse models doubled the number of offspring per litter. Although selected for a primarily female-trait of high fertility (increased litter size), we were interested whether
Marten Michaelis et al.
BMC genomics, 18(1), 889-889 (2017-11-22)
Many genes important for reproductive performance are shared by both sexes. However, fecundity indices are primarily based on female parameters such as litter size. We examined a fertility mouse line (FL2), which has a considerably increased number of offspring and
Uğur Şen et al.
Asian-Australasian journal of animal sciences, 31(6), 827-834 (2017-12-23)
The aim of this study was to compare the effects of 36.5°C and 38.5°C incubation temperatures on the maturation of bovine oocytes and developmental competence of embryos. In experiment 1, oocytes were maturated in bicarbonate-buffered TCM-199 for 22 hours in
Wenjia Li et al.
Cell death & disease, 11(7), 599-599 (2020-08-01)
Alpha-lipoic acid (α-LA), a well-known antioxidant, was proved to active ALDH2 in nitrate tolerance and diabetic animal model. However, the therapeutic advantage of α-LA for heart failure and related signaling pathway have not been explored. This study was designed to
Duo Xu et al.
Cell death & disease, 12(4), 406-406 (2021-04-17)
Escape from programmed cell death is a hallmark of cancer. In this study, we investigated the anti-apoptotic mechanisms and explored the therapeutic potential of BCL-2 homology domain-3 (BH3) mimetics in malignant pleural mesothelioma (MPM), a lethal thoracic malignancy with an
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
相关内容
Discover the formulation for Medium 199 for cell culture applications, including vaccine development.
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