Merck
CN

M8403

Sigma-Aldrich

McCoy 5A培养基

Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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别名:
5A basal medium
EC 号:
MDL编号:
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

运输

ambient

储存温度

2-8°C

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一般描述

McCoy 5A培养基适用于各种组织细胞原代培养。推荐用于在原代细胞培养中生产病毒。

应用

McCoy 5A培养基已用于维持:

  • G-361黑色素瘤细胞;
  • 87-MG细胞
  • 人结肠癌细胞系HCT116

生化/生理作用

McCoys 5A培养基起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。

重悬

补充 0.22 g/L L-谷氨酰胺。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Seungman Park et al.
Scientific reports, 9(1), 13286-13286 (2019-09-19)
We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted
Huw D Summers et al.
ACS nano, 7(7), 6129-6137 (2013-06-19)
Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can
Jon A Read et al.
ACS chemical biology, 14(10), 2134-2140 (2019-09-17)
Enhancer of zeste homologue 2 (EZH2), the catalytic subunit of polycomb repressive complex 2 (PRC2), regulates chromatin state and gene expression by methylating histone H3 lysine 27. EZH2 is overexpressed or mutated in various hematological malignancies and solid cancers. Our
Katsuhiro Uzawa et al.
Scientific reports, 4, 5433-5433 (2014-06-26)
No definitive therapy exists to treat human metastatic tumors. We reported previously that down-regulation of Lin-7C is essential for metastasis of human squamous cell carcinomas (hSCCs). In this study, we investigated the chemical restoration of Lin-7C expression and demonstrated its
A-P Sappino et al.
Oncogenesis, 1, e7-e7 (2012-01-01)
The p53 tumor-suppressor protein has a key role in the induction of cellular senescence, an important barrier to cancer development. However, very little is known about the physiological mediators of cellular senescence induced by p53. CEACAM1 is an immunoglobulin superfamily

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McCoy's 5A Modified Media Formulation

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