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Merck
CN

M8662

Sigma-Aldrich

矿物油

PCR Reagent

别名:

Mineral oil for PCR

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关于此项目

化学文摘社编号:
8042-47-5
EC 号:
232-455-8
MDL编号:
MFCD00131611
UNSPSC代码:
12181504
NACRES:
NA.52

主要文件

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质量水平

100

表单

liquid

包装

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

技术

PCR: suitable

颜色

colorless

折射率

n20/D 1.467 (lit.)

密度

0.84 g/mL at 25 °C (lit.)

异质活性

DNase, RNase, protease, none detected

储存温度

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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相关类别

一般描述

矿物油可用作聚合酶链反应（PCR） 中的反应混合物覆盖层，以防止在没有加热盖的 PCR 仪器的热循环过程中反应溶液蒸发。

应用

矿物油已用于:
  • 常规PCR扩增
  • 逆转录聚合酶链反应 （RT–PCR） 扩增工艺，提高设备和循环仪之间的导热性
  • 防止在 Bravo 自动液体处理平台上进行的单细胞 MATQ 测序过程中蒸发

特点和优势

  • 以5 × 1.5 mL（1 瓶）包装规格提供，便于使用
  • 经测试不含 RNase、DNase和蛋白酶。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

No data available

闪点(°C)

No data available

个人防护装备

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCW2105
MKCN0398
MKCK1218
MKCF5776
MKCC0168

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Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
Klenner MA, et al.
Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
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实验方案

使用Jumpstart™Taq DNA聚合酶(D9307)扩增DNA

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

相关内容

KOD One™ PCR Master Mix for Ultra-Fast, High-Fidelity PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

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