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Merck
CN

M8787

氯化镁 溶液

PCR Reagent, 25 mM MgCI2 solution for PCR

别名:

PCR optimization

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关于此项目

线性分子式:
MgCl2
化学文摘社编号:
7786-30-3
分子量:
95.21
NACRES:
NA.52
PubChem Substance ID:
57654422
UNSPSC Code:
12352302
MDL number:
MFCD00011106
Form:
liquid

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产品名称

氯化镁 溶液, PCR Reagent, 25 mM MgCI2 solution for PCR

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

SMILES string

Cl[Mg]Cl

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

form

liquid

packaging

vial of 1.5 mL

concentration

25 mM±1 mM

technique(s)

PCR: suitable

color

colorless

application(s)

agriculture
diagnostic assay manufacturing

foreign activity

DNase, RNase, none detected

storage temp.

2-8°C

Quality Level

200
300

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相关类别

Application

氯化镁溶液已用作以下材料的组分：

  • 聚合酶链式反应（PCR）扩增混合液
  • 简单序列重复(ISSR)-PCR扩增混合液
  • 16S-23S rRNA基因内转录间隔区（ITS）PCR扩增及限制性内切分析（ITS-PCR- rflp）反应缓冲液
  • 用于定量逆转录-聚合酶链反应（qRT PCR）的PCR反应混合物，以扩增逆转录cDNA
  • 用于RT-PCR检测基因表达的反应混合物
适用于聚合酶链反应的优化。

General description

氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加，镁缺乏会导致产量降低。

存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000308195
0000308195
0000472859
0000472859
0000444896

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Molecular characterization of Fusarium oxysporum f. sp. cubense isolates from banana
Das A, et al.
Pest Management in Horticultural Ecosystems , 18(2), 171-178 (2012)
Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
Adewumi G A, et al.
Food Biotechnology, 28(3), 250-268 (2014)
Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Kadri Õunap et al.
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
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