M8883
4-甲基伞形酮磷酸酯
phosphatase substrate, fluorogenic, ≥98% (HPLC), powder
别名:
4-甲基伞形基磷酸
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关于此项目
经验公式（希尔记法）:
C10H9O6P
化学文摘社编号:
分子量:
256.15
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
222-137-7
MDL number:
Beilstein/REAXYS Number:
1687229
产品名称
4-甲基伞形酮磷酸酯,
InChI key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
InChI
1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
SMILES string
CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
Quality Level
assay
≥98% (HPLC)
form
powder
solubility
water: 25 mg/mL, clear to very slightly hazy, colorless
fluorescence
λex 319 nm; λem 384 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)
storage temp.
−20°C
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General description
磷酸酶的荧光底物。
Application
4-甲基伞形酮磷酸酯可在肽结合试验中作为碱性磷酸酶的作用底物。也用作酶联免疫吸附测定(ELISA)的荧光底物。
Biochem/physiol Actions
4-甲基伞形酮磷酸酯可作为钙调蛋白依赖性磷酸酶的荧光底物，也可用于碱性磷酸酶的动力学研究。在酶联免疫吸附测定(ELISA)中，可作为碱性磷酸酶作用底物。在人免疫缺陷型病毒抗体的酶免疫分析中，作为底物的4-甲基伞形酮磷酸酯比酚酞单磷酸酯灵敏7倍，比对硝基苯磷酸酯灵敏8-13倍。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Maya Heath et al.
JAMA network open, 2(11), e1914996-e1914996 (2019-11-09)
Necrotizing enterocolitis (NEC) in preterm infants is an often-fatal gastrointestinal tract emergency. A robust NEC biomarker that is not confounded by sepsis could improve bedside management, lead to lower morbidity and mortality, and permit patient selection in randomized clinical trials
Ryota Fujii et al.
Biochimica et biophysica acta, 1631(2), 197-205 (2003-03-14)
The fluorescent organophosphorus esters, diethyl 4-methylumbelliferyl phosphate (1), ethyl hexyl 4-methylumbelliferyl phosphate (2) and ethyl 4-methylumbelliferyl heptylphosphonate (3) have been synthesized and evaluated as a sensitive active-site titrant of lipase. The phosphorus esters 1, 2 and 3 inactivated the lipase
Emna Hannachi et al.
Biotechnology reports (Amsterdam, Netherlands), 23, e00360-e00360 (2019-07-26)
The purpose of this study was to implement a fluorometric method for enhancing the detection sensitivity of Toxoplasma gondii in biological fluids. To address this challenge, we designed and produced a recombinant immunoconjugate tool based on a single-chain antibody fragment
Andréa Toma et al.
Diabetes, 58(2), 394-402 (2008-11-18)
A restricted region of proinsulin located in the B chain and adjacent region of C-peptide has been shown to contain numerous candidate epitopes recognized by CD8(+) T-cells. Our objective is to characterize HLA class I-restricted epitopes located within the preproinsulin
C J Reynolds et al.
Scientific reports, 8(1), 672-672 (2018-01-14)
Zika virus (ZIKV) Infection has several outcomes from asymptomatic exposure to rash, conjunctivitis, Guillain-Barré syndrome or congenital Zika syndrome. Analysis of ZIKV immunity is confounded by the fact that several related Flaviviruses infect humans, including Dengue virus 1-4, West Nile
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