M9004
苹果酸脱氢酶 来源于牛心脏
ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)
别名:
L -苹果酸盐：NAD + 氧化还原酶, MDH
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关于此项目
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
Specific activity:
2000-4000 units/mg protein (modified Warburg-Christian)
Biological source:
bovine heart
产品名称
苹果酸脱氢酶 来源于牛心脏, ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)
biological source
bovine heart
form
ammonium sulfate suspension
specific activity
2000-4000 units/mg protein (modified Warburg-Christian)
mol wt
35 kDa
impurities
≤0.01% Glutamic-Oxalacetic Transaminase
≤0.01% Glutamic-Pyruvic Transaminase
storage temp.
2-8°C
Quality Level
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Application
苹果酸脱氢酶已用于评估流动注射系统的研究，用于在线监测生物过程中的富马酸。 它也曾被用于一项研究，调查一种寄生在德州花生上的根结线虫。
General description
牛心脏的苹果酸脱氢酶在 NAD 结合活性位点包含一个组氨酸残基，该残基对活性至关重要。当该组氨酸突变时，观察到活性丧失。
苹果酸脱氢酶是一种细胞质同工酶，是三羧酸循环中的重要催化剂。
Other Notes
在pH7.5,25℃条件下，一个单位每分钟可将1.0 μmol的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。
Physical form
3 M (NH4)2SO4 - 0.01 M KH2PO4 溶液 (pH 7.3) 中的混悬液
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Eisaku Hokazono et al.
Clinica chimica acta; international journal of clinical chemistry, 412(15-16), 1436-1440 (2011-05-10)
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Rhee, J.
Analytica Chimica Acta, 499, 71-80 (2003)
Isoelectric points and molecular weights of proteins: a new table.
Righetti, P.G., and Tudor, G.
Journal of Chromatography A, 220, 115-194 (1981)
Z Y Chen et al.
Plant physiology, 112(2), 677-684 (1996-10-01)
At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One
Vladimir B Ritov et al.
Analytical biochemistry, 333(1), 27-38 (2004-09-08)
This study addresses an application of pyridine nucleotide enzymatic analyses to evaluate the activity of the mitochondrial electron transport chain (reduced nicotinamide adenine dinucleotide (NADH) oxidase) and Complexes I and II in samples of human muscle as small as approximately
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
实验方案
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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