M9163
培养基 199
With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X
别名:
M199 Medium, TCM 199
产品名称
培养基 199, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
浓度
10 ×
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no
运输
ambient
储存温度
2-8°C
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一般描述
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
制备说明
补充0.1 g/L L-谷氨酰胺，0.35 g/L碳酸氢钠（1×）。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Wei Qi et al.
Journal of pharmaceutical sciences, 107(8), 2070-2078 (2018-05-02)
Stable formulations of inactivated polio vaccine (IPV) could reduce cold-chain requirements and increase distribution of the vaccine to developing countries. Recently, significant improvement in thermal stability of IPV vaccines has been achieved by including urea in lyophilized formulations. In the
Xiaofeng Yang et al.
Bioscience reports, 40(10) (2020-09-29)
Transplantion of bone marrow-derived endothelial progenitor cells (EPCs) may be a novel treatment for deep venous thrombosis (DVT). The present study probed into the role of microRNA (miR)-361-5p in EPCs and DVT recanalization. EPCs were isolated from male Sprague-Dawley (SD)
Sujoy Dutta et al.
Eukaryotic cell, 7(7), 1146-1157 (2008-05-20)
Inherent deficiencies of Leishmania in heme biosynthesis were genetically complemented for delta-aminolevulinate-inducible biosynthesis and accumulation of light-excitable uroporphyrin. The phototoxic flagellar immobilization and cytolysis phenotypes and porphyrin mobilization noted previously were further analyzed biochemically and cytologically to delineate the mechanism
Carla Maia et al.
PLoS neglected tropical diseases, 5(6), e1181-e1181 (2011-06-23)
We quantified Leishmania infantum parasites transmitted by natural vectors for the first time. Both L. infantum strains studied, dermotropic CUK3 and viscerotropic IMT373, developed well in Phlebotomus perniciosus and Lutzomyia longipalpis. They produced heavy late-stage infection and colonized the stomodeal
Wen-Xing Ding et al.
Molecular cancer therapeutics, 8(7), 2036-2045 (2009-07-09)
The proteasome and the autophagy systems are two evolutionarily conserved mechanisms for degrading intracellular materials. They are functionally coupled and suppression of the proteasome promotes autophagy. Although suppression of the proteasome leads to cell death, suppression of autophagy can be
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
相关内容
Discover the formulation for Medium 199 for cell culture applications, including vaccine development.
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