M9163
培养基 199
With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X
别名:
M199 Medium, TCM 199
产品名称
培养基 199, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
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General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Preparation Note
补充0.1 g/L L-谷氨酰胺，0.35 g/L碳酸氢钠（1×）。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Wei Qi et al.
Journal of pharmaceutical sciences, 107(8), 2070-2078 (2018-05-02)
Stable formulations of inactivated polio vaccine (IPV) could reduce cold-chain requirements and increase distribution of the vaccine to developing countries. Recently, significant improvement in thermal stability of IPV vaccines has been achieved by including urea in lyophilized formulations. In the
Kai Wang et al.
PloS one, 6(7), e22197-e22197 (2011-07-30)
Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive. In this study, we analyzed the
C Ling et al.
Diabetologia, 51(4), 615-622 (2008-02-14)
Insulin secretion in pancreatic islets is dependent upon mitochondrial function and production of ATP. The transcriptional coactivator peroxisome proliferator activated receptor gamma coactivator-1 alpha (protein PGC-1alpha; gene PPARGC1A) is a master regulator of mitochondrial genes and its expression is decreased
Xiaofeng Yang et al.
Bioscience reports, 40(10) (2020-09-29)
Transplantion of bone marrow-derived endothelial progenitor cells (EPCs) may be a novel treatment for deep venous thrombosis (DVT). The present study probed into the role of microRNA (miR)-361-5p in EPCs and DVT recanalization. EPCs were isolated from male Sprague-Dawley (SD)
Sujoy Dutta et al.
Eukaryotic cell, 7(7), 1146-1157 (2008-05-20)
Inherent deficiencies of Leishmania in heme biosynthesis were genetically complemented for delta-aminolevulinate-inducible biosynthesis and accumulation of light-excitable uroporphyrin. The phototoxic flagellar immobilization and cytolysis phenotypes and porphyrin mobilization noted previously were further analyzed biochemically and cytologically to delineate the mechanism
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
相关内容
Discover the formulation for Medium 199 for cell culture applications, including vaccine development.
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