β-Mannosidase from Helix pomatia

β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.

β-Mannosidase from Helix pomatia has been used:

• in the digestion of glycans for enzymatic analysis
• in the treatment of labeled monogalactosyl Manno-oligosaccharides
• in non-mammalian glycosidase inhibition assays

β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.

One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.

Suspension in 3.0 M (NH₄)₂SO₄ containing 10 mM sodium acetate, pH approx. 4.0