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M9901

变溶菌素来源于球孢链霉菌 ATCC 21553

Name: 变溶菌素 来源于<I>球孢链霉菌 </I> ATCC 21553
Brand: SIGMA

lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified

别名:

Mutanolysin

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关于此项目

化学文摘社编号:

55466-22-3

UNSPSC Code:

12352204

NACRES:

NA.54

MDL number:

MFCD00131635

Specific activity:

≥4000 units/mg protein (biuret)

Biological source:

bacterial (Streptomyces globisporus)

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biological source

bacterial (Streptomyces globisporus)

Quality Level

300

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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General description

从 链霉菌 中提取的变溶菌素由两种主要的裂解酶组成，可能是一种有效的龋病防治剂。

Application

诱变溶菌素在一项研究中被用于评估牛奶 链球菌的裂解和原生质体的生成。它也在一项研究中被用于研究研究B类 链球菌 向原生质体的转化。它为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。它已可用于形成球状体以分离DNA。

为从细菌分离中易于降解的生物分子和RNA提供了温和的细胞裂解。它已可用于形成球状体以分离DNA。

Biochem/physiol Actions

变溶菌素可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。

变溶菌素是一种N-乙酰胞壁质酶。同溶菌酶一样是一种胞壁水解酶，可切开细菌细胞壁中肽聚糖-多糖聚合物的β-N-乙酰胞壁酸-(1→4)-N-乙酰葡糖胺的连键。其羧基末端部分参与识别和结合独特的细胞壁聚合物。变溶菌素可裂解李斯特菌和其他革兰氏阳性菌，如乳杆菌属和乳球菌属。

Physical form

冻干粉，含Ficoll^® 和琥珀酸钠缓冲盐

Other Notes

以粪链球菌细胞壁混悬液为底物，在1mL体积中，1单位变溶菌素在pH 6.0、37 °C条件下每分钟可产生0.01的ΔA₆₀₀。

有关复合碳水化合物分析酶的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

Legal Information

Ficoll is a registered trademark of Cytiva

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

存储类别

13 - Non Combustible Solids

wgk

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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Group A Streptococcus M1T1 Intracellular Infection of Primary Tonsil Epithelial Cells Dampens Levels of Secreted IL-8 Through the Action of SpyCEP.

Amelia T Soderholm et al.

Frontiers in cellular and infection microbiology, 8, 160-160 (2018-06-06)

Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host

β-Barrels covalently link peptidoglycan and the outer membrane in the α-proteobacterium Brucella abortus.

Pierre Godessart et al.

Nature microbiology, 6(1), 27-33 (2020-11-04)

Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial

Lysis and protoplast formation of group B streptococci by mutanolysin.

G B Calandra et al.

Infection and immunity, 28(3), 1033-1037 (1980-06-01)

Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls

Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era.

Helena Žemličková et al.

Journal of medical microbiology, 67(7), 1003-1011 (2018-06-02)

Purpose. The aim of this study was to characterize serogroup 19 isolates resistant to macrolides and/or penicillin found among pneumococci recovered from cases of invasive and respiratory tract disease in the Czech Republic in 2014.Methods. Pneumococcal isolates of serotypes 19A

Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci

Kondo, J. and L. McKay

Journal of Dairy Science, 65, 1428-1431 (1982)

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实验方案

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

GenElute™细菌基因组DNA试剂盒实验方案（NA2100、NA2110、NA2120）

的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。

变溶菌素的酶促检测

在测定变溶菌素活性时，使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。

全球贸易项目编号

货号	GTIN
M9901-1KU	04061835557806
M9901-10KU	04061835557790
M9901-50KU	04061835557813
M9901-5KU	04061835557820

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