用途
sufficient for 100 colorimetric or fluorometric tests
检测方法
colorimetric
fluorometric
储存温度
−20°C
一般描述
丙氨酸是一种非必需氨基酸，在肌肉组织和肝脏之间的葡萄糖-丙氨酸循环中起关键作用。在氨基酸降解组织（如肌肉）中，氨基通过转氨反应汇集为谷氨酸。谷氨酸的氨基通过丙氨酸氨基转移酶转移到丙酮酸，形成丙氨酸和 α-酮戊二酸。丙氨酸进入血液并转运至肝脏。该反应在肝脏中逆转，丙酮酸盐可用于糖异生形成葡萄糖 ，葡萄糖可通过循环系统返回至其他组织。丙氨酸水平升高与高血压、能量摄入、胆固醇水平和体重指数相关。
应用
丙氨酸检测试剂盒已用于测定细胞内的丙氨酸池。
适用于细胞和组织培养上清、尿液、血浆、血清等生物样本中的丙氨酸检测。
生化/生理作用
在本试剂盒中，丙氨酸浓度由偶联酶分析法测定，其结果为比色法 (570 nm)/荧光法（λex = 535/λ em = 587 nm）乘积，与存在的丙氨酸成比例。本试剂盒的典型检测范围为 2-10 nmole（比色法）和 0.2-1 nmole（荧光测定法）。
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
GABA transaminases from Saccharomyces cerevisiae and Arabidopsis thaliana complement function in cytosol and mitochondria.
Cao J, et al.
Yeast, 30(7), 279-289 (2013)
Berg J M, et al.
Biochemistry (5th Edition) (2002)
Dmitry Frank et al.
Neuropharmacology, 155, 173-184 (2019-06-04)
Post-stroke depression (PSD) is a common and serious complication following stroke. Both stroke and depression have independently been associated with pathologically elevated glutamate levels in the brain's extra-cerebral fluid (ECF). Here we evaluate an alternative therapeutic approach to PSD with
Xiaogang Li et al.
iScience, 23(12), 101763-101763 (2020-12-01)
Regulation of glucose homeostasis is a fundamental process to maintain blood glucose at a physiological level, and its dysregulation is associated with the development of several metabolic diseases. Here, we report on a zebrafish mutant for Aldo-keto-reductase 1a1b (akr1a1b) as a
Deanna N Edwards et al.
The Journal of clinical investigation, 131(4) (2020-12-16)
Rapidly proliferating tumor and immune cells need metabolic programs that support energy and biomass production. The amino acid glutamine is consumed by effector T cells and glutamine-addicted triple-negative breast cancer (TNBC) cells, suggesting that a metabolic competition for glutamine may
商品
本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物，但许多肿瘤细胞表现出线粒体活性增加。
Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
实验方案
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
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