usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer; hematological disorder
storage temp.
−20°C
Gene Information
human ... H6PD(9563)
mouse ... H6PD(100198)
rat ... H6PD(298655)
General description
葡萄糖-6-磷酸脱氢酶（G6PDH）可催化葡萄糖-6-磷酸转化为6-磷酸葡糖酸-δ-内酯，这是磷酸戊糖途径（PPP）的第一步和限速步骤。PPP途径对于维持辅酶烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和戊糖的产生至关重要。所产生的NADPH对于通过GSH再生进行氧化还原调节，以及为脂肪酸生物合成提供还原当量至关重要。G6PDH的缺陷使个体易患非免疫性溶血性贫血。
Application
葡萄糖-6-磷酸脱氢酶测定试剂盒已用于测定葡萄糖-6-磷酸脱氢酶（G6PDH）的活性 ，以及PDH（丙酮酸脱氢酶）的测量。
适用于测定各种组织和细胞中葡萄糖-6-磷酸脱氢酶（G6PDH）的活性。
Biochem/physiol Actions
在葡萄糖-6-磷酸脱氢酶测定试剂盒中，葡萄糖-6-磷酸被氧化以产生能够通过比色（450nm）法进行测定的特异性检测产物。G6PDH检测试剂盒可检测出每孔低至0.04mU 的G6PDH活性。
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
存储类别
8A - Combustible corrosive hazardous materials
法规信息
常规特殊物品
此项目有
Jeanine Montano et al.
Frontiers in microbiology, 11, 6-6 (2020-02-11)
Contamination of edible produce leaves with human bacterial pathogens has been associated with serious disease outbreaks and has become a major public health concern affecting all aspects of the market, from farmers to consumers. While pathogen populations residing on the
Sandra Meyer et al.
Nutrients, 12(4) (2020-04-08)
The present study tested the hypothesis that the liver lipid-lowering effect of insect meal (IM) is caused by its low methionine concentration. A total of fifty, male obese Zucker rats were randomly assigned to five groups of 10 rats each
Chhanda Biswas et al.
The Journal of biological chemistry, 289(39), 26882-26894 (2014-08-12)
With oxidative injury as well as in some solid tumors and myeloid leukemia cells, heme oxygenase-1 (HO-1), the anti-oxidant, anti-inflammatory, and anti-apoptotic microsomal stress protein, migrates to the nucleus in a truncated and enzymatically inactive form. However, the function of
Rapid Elevation in CMPF May Act As a Tipping Point in Diabetes Development
Ying L, et al.
Cell Reports, 14(12), 2889-2900 (2016)
Nuclear Heme Oxygenase-1 (HO-1) Modulates Subcellular Distribution and Activation of Nrf2, Impacting Metabolic and Anti-oxidant Defenses.
Chhanda B, et al.
The Journal of Biological Chemistry, jbc-M114 (2014)
实验方案
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。
相关内容
Instructions
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