usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
neurological disorders; cardiovascular diseases; cancer
storage temp.
2-8°C
Gene Information
human ... ACO1(48), ACO2(50)
mouse ... ACO1(11428), ACO2(11429)
rat ... ACO1(50655), ACO2(79250)
General description
该乌头酸酶是一个含铁-硫的酶家族，其催化柠檬酸异构化为异柠檬酸。人们已经在真核生物中鉴定出了由不同基因编码的两种乌头酸酶。乌头酸酶 1（ACO1，c-乌头酸酶）是一种胞质酶，而乌头酸酶 2（ACO2，m-乌头酸酶）是一种在三羧酸循环中起作用的线粒体酶。乌头碱可被氧化应激可逆地失活，并且细胞和组织中的乌头酸酶活性已被用作一种氧化损伤的生物标志物。
Application
乌头酸酶活性检测试剂盒已被用于测量细胞溶质和线粒体顺乌头酸酶的活性。
适用于测量组织和细胞等生物样品中的乌头酸酶活性
Biochem/physiol Actions
乌头酸酶活性检测试剂盒提供了一种测量各种样品中的乌头酸酶活性的简单、直接的方法。在偶联酶反应中测定乌头酸酶活性，在该反应中柠檬酸盐被乌头酸酶转化为了异柠檬酸。因此，比色（450nm）产物与酶活性的量成正比。一个单位的乌头酸酶是指在 25℃，pH 7.4条件下每分钟将异柠檬酸异构化产生 1.0 μmole 柠檬酸盐的酶量。
Features and Benefits
兼容高通量处理系统。
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
存储类别
8A - Combustible corrosive hazardous materials
法规信息
低风险生物材料
此项目有
Jayasimman Rajendran et al.
EMBO molecular medicine, 11(1) (2018-12-12)
Alternative oxidase (AOX) is a non-mammalian enzyme that can bypass blockade of the complex III-IV segment of the respiratory chain (RC). We crossed a Ciona intestinalis AOX transgene into RC complex III (cIII)-deficient Bcs1l
Vincenzo Maione et al.
Biochimica et biophysica acta. General subjects, 1862(9), 1980-1987 (2018-05-31)
The CIA2A protein, in complex with CIAO1, has been proposed to be exclusively implicated in the maturation of cytosolic aconitase. However, how the CIA2A-CIAO1 complex generates active aconitase is still unknown and the available structural information has not provided any
Evaluation of the adaptogenic potential exerted by ginsenosides Rb1 and Rg1 against oxidative stress-mediated neurotoxicity in an in vitro neuronal model.
Carlos F M, et al.
PLoS ONE, 12(8), e0182933-e0182933 (2017)
Spyridon Gourdoupis et al.
Journal of the American Chemical Society, 140(43), 14401-14412 (2018-10-03)
The maturation of mitochondrial iron-sulfur proteins requires a complex protein machinery. Human IBA57 protein was proposed to act in a late phase of this machinery, along with GLRX5, ISCA1, and ISCA2. However, a molecular picture on how these proteins cooperate
NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis.
Alvarez S W, et al.
Nature, 551(7682), 639-639 (2017)
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