Merck
CN

MAK057

Sigma-Aldrich

柠檬酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

应用

cosmetics
food and beverages

检测方法

colorimetric
fluorometric

相关疾病

cancer

储存温度

−20°C

一般描述

柠檬酸是三羧酸(TCA)循环的关键中间体,通过向乙酰辅酶A的乙酰基中加入草酰乙酸而得。柠檬酸通过柠檬酸-苹果酸梭子从线粒体中转运出来,然后转化回乙酰辅酶A进行脂肪酸合成。柠檬酸是脂肪酸合成和糖酵解的别构调节剂,分别通过作用于乙酰辅酶A羧化酶和磷酸果糖激酶来实现。由于性别、年龄和各种其他因素(包括疾病状态),柠檬酸的代谢和分布差异可能很大。前列腺癌细胞中的细胞柠檬酸水平降低,柠檬酸水平可能是前列腺癌生长速率的标志物。柠檬酸检测试剂盒提供了一种可简单、灵敏、快速地对各种样品中的柠檬酸进行定量的方法。

应用

柠檬酸检测试剂盒用于检测样品中柠檬酸的浓度。

适用性

适用于对包括组织和细胞在内的多种样品中的柠檬酸盐进行测定

原理

柠檬酸的浓度通过酶偶联法检测,产生与存在的柠檬酸成比例的比色(570 nm)/荧光(λex = 535/λem = 587 nm)产物。柠檬酸检测试剂盒的灵敏度在各种样品中通常为0.1至10 nM(2 μmM-10 mM)柠檬酸。

相关产品

产品编号
说明
价格

危险声明

预防措施声明

危险分类

Aquatic Chronic 3

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

法规信息

常规特殊物品

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High-intensity sprint training inhibits mitochondrial respiration through aconitase inactivation.
Larsen F J, et al.
Faseb Journal, 30(1), 417-427 (2015)
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Scientific reports, 9(1), 11374-11374 (2019-08-08)
Vascular calcification is highly prevalent in patients with chronic hemodialysis. Increased acetatemia during hemodialysis sessions using acetate-acidified bicarbonate has also been associated with several abnormalities, By contrast, these abnormalities were not induced by citrate-acidified bicarbonate dialysis. Moreover, citrate is biocompatible
Nimrit Goraya et al.
Kidney international, 95(5), 1190-1196 (2019-03-09)
Acid (H+) retention appears to contribute to progressive decline in glomerular filtration rate (GFR) in patients with chronic kidney disease (CKD), including some patients without metabolic acidosis. Identification of patients with H+ retention but without metabolic acidosis could facilitate targeted
Jade D Bailey et al.
Cell reports, 28(1), 218-230 (2019-07-04)
Classical activation of macrophages (M(LPS+IFNγ)) elicits the expression of inducible nitric oxide synthase (iNOS), generating large amounts of NO and inhibiting mitochondrial respiration. Upregulation of glycolysis and a disrupted tricarboxylic acid (TCA) cycle underpin this switch to a pro-inflammatory phenotype.
Di Zhu et al.
Science advances, 6(31), eabb2529-eabb2529 (2020-08-14)
Mild mitochondrial stress experienced early in life can have beneficial effects on the life span of organisms through epigenetic regulations. Here, we report that acetyl-coenzyme A (CoA) represents a critical mitochondrial signal to regulate aging through the chromatin remodeling and

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