usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
葡萄糖是大多数细胞的主要能量来源。葡萄糖的细胞摄取是葡萄糖代谢的首个限速步骤,该过程受到严密的调控。GLUT家族的转运蛋白可促进葡萄糖摄取，而这些蛋白的表达和活性受到多种机制的调控。许多癌细胞的葡萄糖摄取增加，表现出高速率的有氧糖酵解。胰岛素耐受性细胞在遇到胰岛素刺激时,葡萄糖摄取会减弱。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
Application
葡萄糖摄取检测试剂盒（比色法）已被用于测量各种细胞的葡萄糖摄取。
适用于检测在96孔微量滴定板中培养的贴壁细胞或悬浮细胞的葡萄糖摄取。
Biochem/physiol Actions
葡萄糖摄取检测试剂盒（比色法）可简单、直接地测定多种细胞的葡萄糖摄取能力。该试剂盒使用葡萄糖类似物2-脱氧葡萄糖（2-DG）测量葡萄糖摄取，2-DG在测定中被细胞摄取并被己糖激酶磷酸化为2-DG6P。2-DG6P无法进入后续代谢步骤，因而会在细胞中聚集，直接成比例地反映细胞的葡萄糖摄取水平。在该测定中,2-DG的摄取水平通过偶联酶促反应方法测定,其中2-DG6P在此过程中被氧化生成NADPH，随后通过再循环扩增反应来测定NAPDH浓度,而后一反应中谷胱甘肽还原酶将利用NADPH生成谷胱甘肽。谷胱甘肽产物与DTNB反应生成TNB，后者可在412 nm波长处检测到。
存储类别
10 - Combustible liquids
signalword
Danger
wgk
WGK 3
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1
法规信息
监管及禁止进口产品
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