usage
sufficient for 100 fluorometric tests
detection method
fluorometric
storage temp.
−20°C
Gene Information
human ... DPP4(1803)
mouse ... DPP4(13482)
rat ... DPP4(25253)
General description
二肽基肽酶-4（DPP4），也称为 CD26 和腺苷脱氨酶复合蛋白 2，是丝氨酸外肽酶，其从多肽的 N-末端切割 X-脯氨酸和 X-丙氨酸残基。DPP4 是跨膜糖蛋白，其活性调节多种肽（例如生长因子、趋化因子和神经肽）的生物活性。DPP4 通过调节胰高血糖素样肽-1在葡萄糖代谢中起主要作用，并且 DPP4 抑制剂通常用于治疗 2 型糖尿病。DPP4 在免疫调节中也起重要作用，可能在肿瘤抑制中发挥作用。DPP4 基因的缺失会影响碳水化合物的代谢，从而导致精神发育迟滞和肌张力减退。在肝再生、肝硬化和肝肿瘤发生过程中观察到蛋白质的异常表达。
Application
DPP4 活性检测试剂盒已用于检测匀浆中二肽基肽酶-4（DPP4）的活性。
适用于多种样本（包括组织、细胞和血清）中二肽基肽酶-4(DPP4)的测定
Biochem/physiol Actions
DPP4 活性检测试剂盒提供了一种简单直接的方法，用于测量各种样品中的 DPP4 活性。在该检测中，DPP4 切割非荧光底物 H-Gly-Pro-AMC 以释放荧光产物 7-氨基-4-甲基香豆素（AMC）（λex = 360/λem = 460 nm）。一个单位的 DPP4 是在 37℃ 下，每分钟水解 DPP4 底物，以产生 1.0 μmole 的 AMC 的酶量。
Features and Benefits
兼容高通量处理系统。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
法规信息
监管及禁止进口产品
此项目有
Molecular analyses of human and rat dipeptidyl peptidase IV.
Abbott C A, et al.
Cellular peptidases in immune functions and diseases, 161-169 (1997)
Eri Watanabe et al.
Angiogenesis, 23(4), 667-684 (2020-07-24)
Platelet-derived growth factor-B (PDGF-B) is a main factor to promote adipose tissue angiogenesis, which is responsible for the tissue expansion in obesity. In this process, PDGF-B induces the dissociation of pericytes from blood vessels; however, its regulatory mechanism remains unclear.
Active phytochemicals of Pueraria tuberosa for DPP-IV inhibition: in silico and experimental approach.
Srivastava S, et al.
Journal of diabetes and metabolic disorders, 16(1), 46-46 (2017)
Yi-Chen Li et al.
Communications biology, 4(1), 144-144 (2021-01-31)
Peritoneal dialysis (PD) possesses multiple advantages for end stage renal disease. However, long-term PD triggers peritoneal fibrosis (PF). From the nationwide analysis of diabetic PD patients (n = 19,828), we identified the incidence of PD failure was significantly lower in diabetic patients
Shivani Srivastava et al.
Journal of diabetes and metabolic disorders, 16, 46-46 (2017-12-05)
We had earlier reported that the extract of Pueraria tuberosa significantly inhibits DPP-IV enzyme, resulting in glucose tolerance response in rats. In this study, we have explored the active phytochemicals responsible for this potential. The results have been validated in
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