Merck
CN

MAK113

Sigma-Aldrich

ATP酶/ GTP酶活性检测试剂盒

sufficient for 200 colorimetric tests

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用途

sufficient for 200 colorimetric tests

检测方法

colorimetric

相关疾病

cancer

储存温度

2-8°C

一般描述

ATP酶和GTP酶会催化ATP或GTP分解为ADP或GDP和游离磷酸盐。这些酶在运输、信号转导、蛋白质生物合成和细胞分化中起关键作用。

特点和优势

兼容高通量的操作系统。

适用性

适于测定ATP酶和GTP酶的活性,也可用于ATP酶和GTP酶抑制剂的筛选试验。

原理

ATPase / GTPase活性检测试剂盒提供了一种简、单直接的以微孔板形式测量ATP酶 / GTP酶活性的方法。该试剂盒使用单一试剂制剂即可在室温下在30分钟内准确测定酶活性。孔雀石绿试剂与酶释放的游离磷酸盐形成稳定的深绿色,产生可在620 nm(600-660 nm)下测定的、与酶活性成正比的比色产物。一个活性单位是在测定条件下每分钟催化产生1μ摩尔游离磷酸盐的酶量。

象形图

Corrosion

警示用语:

Warning

危险声明

预防措施声明

危险分类

Met. Corr. 1

储存分类代码

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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