usage
sufficient for 100 tests (Ultraviolet)
detection method
colorimetric
relevant disease(s)
cardiovascular diseases; genitourinary disorders; aging/geriatric diseases; orthopedic diseases
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CKB(1152), CKM(1158)
mouse ... CKB(12709), CKM(12715)
rat ... CKB(24264), CKM(24265)
General description
肌酸激酶(CK),又称磷酸肌酸激酶,是催化一个磷酸基团从ATP转移到肌酸,产生磷酸肌酸的酶,磷酸肌酸是肌肉和脑组织的重要能量储备。CK是一种由B(大脑)和M(肌肉)亚基组成的二聚体蛋白。现已发现三种同工酶,即:CK-MM、CK-MB和CK-BB。在心肌梗塞、横纹肌溶解、肌营养不良和肾功能衰竭等各种病理状况下,CK水平升高。
Application
适用于检测各种样品中(如血清和血浆)的肌酸激酶活性。本试剂盒可用于研究各种化合物对肌酸激酶活性的影响。
Biochem/physiol Actions
通过偶联酶反应确定肌酸激酶活性,在340 nm处测量反应产生的NADPH,结果与样品中的CK活性成比例。在此反应中,磷酸肌酸和ADP转化为肌酸和ATP。己糖激酶将产生的ATP用于磷酸化葡萄糖,生成葡萄糖-6-磷酸;在葡萄糖-6-磷酸脱氢酶存在的情况下,NADP氧化葡萄糖-6-磷酸,生成NADPH和6-磷酸-D-葡萄糖酸。一个单位的CK指pH 6.0时,每分钟将1.0μmole磷酸盐从磷酸肌酸转移到ADP的酶量。 该试剂盒的线性范围为30-1,800 单位/L CK活性。
Features and Benefits
兼容高通量的操作系统。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
法规信息
低风险生物材料
常规特殊物品
此项目有
相关内容
Instructions
Marcus Waskiw-Ford et al.
Nutrients, 12(4) (2020-04-16)
Leucine-enriched essential amino acids (LEAAs) acutely enhance post-exercise myofibrillar protein synthesis (MyoPS), which has been suggested to be important for muscle repair and recovery. However, the ability of LEAAs to concurrently enhance MyoPS and muscle damage recovery in free-living humans
P2RX7 Purinoceptor: A Therapeutic Target for Ameliorating the Symptoms of Duchenne Muscular Dystrophy
Sinadinos A
PLoS Medicine null
Crystal structure of rabbit muscle creatine kinase.
Rao JK, et al.
Febs Letters, 133-7 null
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAK116-1KT | 04061834065388 |
