产品名称
磷脂测定试剂盒, sufficient for 100 colorimetric or fluorometric tests
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
food and beverages
pharmaceutical
detection method
colorimetric
fluorometric
shipped in
dry ice
storage temp.
−20°C
Application
适用于生物样品中的磷脂检测。
磷脂测定试剂盒已用于生化分析 和磷脂定量。
Biochem/physiol Actions
在该检测中，磷脂（如卵磷脂、溶血卵磷脂和鞘磷脂）会被酶法水解以释放胆碱，而胆碱则可通过胆碱氧化酶和H2O2-特异性染料所测定。所得的比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物与样品中的磷脂浓度成正比。对于比色测定，检测的线性范围是3 - 200 μM；对于荧光测定，检测的线性范围是0.6 - 20 μM。
Features and Benefits
兼容高通量的操作系统。
General description
磷脂是一种脂质，是构成细胞膜的重要组成部分，在信号转导中起着重要作用。大多数磷脂包含一分子甘油二酯，一分子磷酸基和一分子胆碱。
在该检测中,磷脂（如卵磷脂、溶血卵磷脂和鞘磷脂）会被酶促水解为胆碱，随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物，此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM，荧光法则为0.6 - 20 μM。
在该检测中,磷脂（如卵磷脂、溶血卵磷脂和鞘磷脂）会被酶促水解为胆碱，随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物，此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM，荧光法则为0.6 - 20 μM。
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
存储类别
10 - Combustible liquids
法规信息
含少量动物源组分生物产品
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