用途
sufficient for 100 colorimetric or fluorometric tests
应用
cosmetics
food and beverages
pharmaceutical
检测方法
colorimetric
fluorometric
运输
dry ice
储存温度
−20°C
一般描述
磷脂是一种脂质,是构成细胞膜的重要组成部分,在信号转导中起着重要作用。大多数磷脂包含一分子甘油二酯,一分子磷酸基和一分子胆碱。
在该检测中,磷脂(如卵磷脂、溶血卵磷脂和鞘磷脂)会被酶促水解为胆碱,随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物,此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM,荧光法则为0.6 - 20 μM。
在该检测中,磷脂(如卵磷脂、溶血卵磷脂和鞘磷脂)会被酶促水解为胆碱,随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物,此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM,荧光法则为0.6 - 20 μM。
应用
磷脂测定试剂盒已用于生化分析 和磷脂定量。
适用于生物样品中的磷脂检测。
生化/生理作用
在该检测中,磷脂(如卵磷脂、溶血卵磷脂和鞘磷脂)会被酶法水解以释放胆碱,而胆碱则可通过胆碱氧化酶和H2O2-特异性染料所测定。所得的比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物与样品中的磷脂浓度成正比。对于比色测定,检测的线性范围是3 - 200 μM;对于荧光测定,检测的线性范围是0.6 - 20 μM。
特点和优势
兼容高通量的操作系统。
警示用语:
Danger
危险声明
危险分类
Aquatic Chronic 3 - Resp. Sens. 1
储存分类代码
10 - Combustible liquids
法规信息
含少量动物源组分生物产品
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Kevin M Tharp et al.
American journal of physiology. Gastrointestinal and liver physiology, 310(10), G855-G864 (2016-04-02)
Gallstone disease is a widespread disorder costing billions for annual treatment in the United States. The primary mechanisms underlying gallstone formation are biliary cholesterol supersaturation and gallbladder hypomotility. The relative contribution of these two processes has been difficult to dissect
Epithelial disruption of Gab1 perturbs surfactant homeostasis and predisposes mice to lung injuries.
Wang K, et al.
American Journal of Physiology. Lung Cellular and Molecular Physiology, 311(6), L1149-L1159 (2016)
Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats.
Ibrahim ZS, et al.
Molecular Medicine Reports, 13(4), 3017-3026 (2016)
Prevention of gallbladder hypomotility via FATP2 inhibition protects from lithogenic diet-induced cholelithiasis.
Tharp KM, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 310(10), G855-G864 (2016)
Inhibiting mevalonate pathway enzymes increases stromal cell resilience to a cholesterol-dependent cytolysin.
Griffin S, et al.
Scientific Reports, 7(1), 17050-17050 (2017)
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