Merck
CN

MAK135

Sigma-Aldrich

ADP/ATP 比率检测试剂盒

sufficient for 100 tests (bioluminescent)

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NACRES:
NA.84

用途

sufficient for 100 tests (bioluminescent)

application(s)

pharmaceutical

detection method

chemiluminescent

相关疾病

cancer

储存温度

−20°C

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此商品
MAK133MAK113FLASC
detection method

chemiluminescent

detection method

chemiluminescent

detection method

colorimetric

detection method

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

application(s)

pharmaceutical

application(s)

-

application(s)

-

application(s)

-

relevant disease(s)

cancer

relevant disease(s)

hematological disorder; cancer

relevant disease(s)

cancer

relevant disease(s)

-

一般描述

ADP/ATP比例变化已用于区分细胞死亡和活率模式。ATP水平升高、ADP水平降低表示细胞正在增殖。相反,ATP降低、ADP的升高表示细胞凋亡或坏死,且相对于凋亡,坏死细胞ATP降低和ADP升高程度更为明显。

特点和优势

兼容高通量的操作系统。

适用性

适于检测细胞中的凋亡和坏死情况,或研究化合物对细胞增殖的影响。

原理

ADP/ATP比率测定试剂盒提供了一种简单直接的细胞ADP和ATP水平测定方法,可用于筛查细胞凋亡、坏死和增殖。该测定包括两个步骤。第一步,利用工作试剂裂解细胞以释放ATP和ADP。在存在萤光素酶的条件下,ATP立即与底物D-萤光素反应产生荧光。光强度是细胞内ATP浓度的直接指标。

萤光素酶
ATP + D-萤光素 + O2 ----------> 氧化萤光素 + AMP + PPi + CO2 + 荧光

第二步,ADP通过酶解反应转化为ATP。然后像第一步一样,新形成的ATP与D-荧光素反应。测得的第二个光强度代表样品中的总ADP和ATP浓度。

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危险声明

危险分类

Aquatic Chronic 3 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

法规信息

含少量动物源组分生物产品
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