用途
sufficient for 200 fluorometric tests (orange)
应用
cosmetics
food and beverages
检测方法
fluorometric
相关疾病
cancer; neurological disorders
储存温度
−20°C
一般描述
在有氧呼吸期间氧气减少以及在细胞内各种酶体系的作用下，可生成活性氧类物质（ROS）。在生理水平上，ROS有助于细胞信号传导和宿主防御能力。当ROS生成增加至超过生物系统的解毒能力时，将导致氧化应激和细胞损伤。对细胞的主要损伤来源于ROS诱导的大分子改变，如膜脂质的多不饱和脂肪酸、必需蛋白质和DNA。已有研究证明ROS与疾病状态有关，如阿尔茨海默病、帕金森病、癌症和衰老。
应用
本试剂盒适于通过酶标仪、流式细胞仪或带TRITC滤镜的荧光显微镜检测细胞内的ROS。
细胞内活性氧类物质（ROS）检测试剂盒（荧光法）已可用于评估机械通气（MV）对线粒体的损害。它还被用于测量细胞内ROS的产生。
生化/生理作用
细胞内ROS检测试剂盒（荧光法）是一种灵敏的一步式荧光测定法，仅需1小时孵育即可检测活细胞中的细胞内ROS（特别是超氧化物和羟基自由基）。ROS与细胞可透过性传感器反应，产生的荧光产物（λex = 540/λem = 570 nm）与ROS含量成比例。该测定可使用方便的96孔或384孔微量滴定板。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Neurodegenerative diseases and oxidative stress.
Barnham K J, et al.
Nature Reviews. Drug Discovery, 3(3), 205-205 (2004)
Tao Zheng et al.
Frontiers in pharmacology, 12, 669146-669146 (2021-05-25)
Although miR-327 had a protective effect on cardiomyocytes as described previously, the potential mechanism still needs further exploration. The aim of this study was to investigate the role and mechanism of miR-327 on oxidative stress in myocardial ischemia/reperfusion injury (MI/RI)
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants.
Das K and Roychoudhury A
Frontiers in environmental science., 2, 53-53 (2014)
Feng Gao et al.
Journal of cellular biochemistry, 121(11), 4450-4457 (2020-03-13)
The mechanisms driving the pathologic progression of osteoarthritis (OA) have not yet to be fully elucidated. Excessive and irreversible breakdown of the extracellular matrix is the main hallmark of OA. Inhibitors of DPP-4 have been widely used for over a
Udayakumar Karunakaran et al.
Redox biology, 45, 102029-102029 (2021-06-10)
Prolonged hyperglycemia plays a major role in the progression of β-cell loss in diabetes mellitus. Here we report an insulin sensitizer thiazolidinedione Pioglitazone selectively preserves the beta cells against high glucose-induced dysfunction by activation of AMPK and Glutaminase 1 (GLS1)
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