产品名称
过氧化氢检测试剂盒(荧光法), sufficient for 500 fluorometric tests (infrared fluorescence)
usage
sufficient for 500 fluorometric tests (infrared fluorescence)
detection method
fluorometric
relevant disease(s)
orthopedic diseases; cardiovascular diseases; aging/geriatric diseases; neurological disorders; pulmonary disorders
storage temp.
−20°C
Features and Benefits
本检测方法能够兼容高通量操作系统。
Application
荧光过氧化氢检测试剂盒为用户定量多种不同样本(如细胞提取物及溶液)中的过氧化氢水平提供了简单可靠的检测方法。
过氧化氢检测试剂盒(荧光法)已可用于测定氧化压力(H2O2)。
Biochem/physiol Actions
过氧化氢检测试剂盒(荧光法)还可用于检测活细胞释放的过氧化氢或者由氧化酶活性产生的过氧化氢。该试剂盒利用过氧化物酶底物与过氧化氢反应,产生的红外荧光产物(λex = 640/λem = 680 nm)可用荧光酶标仪进行分析。 在pH 4-10范围内,荧光产物的生成不受pH影响,因此,该试剂盒优于需要在低pH溶液中测定过氧化氢的其他荧光试剂盒,后者的探针灵敏度降低。 红外荧光探针对于由生物样品自发荧光造成的测定背景不太敏感。该测定兼容高通量操作系统。
General description
过氧化氢是由分子氧代谢产生的活性氧类物质,既是一种细胞内信号传导信使,也是一种氧化应激来源。过氧化氢由细胞通过多种机制生成,如中性粒细胞和巨噬细胞内NOX介导的ROS产生(呼吸爆发),或者通过在线粒体呼吸期间由电子泄漏产生的超氧阴离子的歧化酶催化产生ROS。异常的过氧化氢产生将导致细胞氧化损伤以及哮喘、动脉粥样硬化、骨质疏松症和神经变性等疾病进展。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物源性产品
此项目有
The mechanism of iron-compensation for manganese deficiency of Streptococcus pneumoniae.
Cao K, et al.
Journal of Proteomics, 184, 62-70 (2018)
Formation of an angular aromatic polyketide from a linear anthrene precursor via oxidative rearrangement.
Gao G, et al.
Cell Chemical Biology, 24(7), 881-891 (2017)
Miyuki Takashima et al.
Journal of inflammation research, 14, 4053-4067 (2021-08-31)
Milk depression is the major driver of economic loss due to mastitis in dairy animals. The aim of this study was to identify potential mediators of milk depression by investigating the local and systemic changes in gene expression or cytokine
Guixi Gao et al.
Cell chemical biology, 24(7), 881-891 (2017-07-18)
Bacterial aromatic polyketides are a group of natural products synthesized by polyketide synthases (PKSs) that show diverse structures and biological activities. They are structurally subclassified into linear, angular, and discoid aromatic polyketides, the formation of which is commonly determined by
Fritz Schweikart et al.
Analytical chemistry, 91(3), 1748-1751 (2019-01-24)
HPLC coupled to both UV and MS is an established setup for purity assessments in many areas. With evolving technology, instrument sensitivity increases, calling for lower sample concentration, while light flux in a commercial UV detector cell is considerably higher
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持