usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
Gene Information
human ... PDHA1(5160), PDHA2(5161), PDHB(5162), PDHX(8050)
mouse ... PDHA1(18597), PDHA2(18598), PDHB(68263), PDHX(27402)
rat ... PDHA1(29554), PDHA2(117098), PDHB(289950), PDHX(311254)
General description
丙酮酸脱氢酶（PDH）是一种线粒体酶，它可以催化丙酮酸转化为乙酰辅酶a和一氧化碳2，也可以连接三羧酸（TCA）和糖酵解路径。该酶被磷酸化抑制，被去磷酸化激活。PDH突变与丙酮酸脱氢酶缺乏（导致乳酸酸中毒和神经功能障碍）和莱氏症候群有关。 PDH也与癌基因诱导的衰老有关。 PDH测量可以为代谢功能和肿瘤发生提供参考。
Application
PDH活性测定试剂盒已用于测定丙酮酸脱氢酶的活性。
适于测定不同生物样品内的PDH活性，如细胞和组织培养上清液以及纯化所得的线粒体。
Biochem/physiol Actions
丙酮酸脱氢酶活性可以通过偶联酶反应进行测定，生成的比色产物(450 nm)和酶促活性成正比。一个单位的丙酮酸脱氢酶是在37°C下，每分钟产生 1.0毫克分子量NADH的酶的量。
Other Notes
仅试剂盒组分
产品编号
说明
- PDH Assay Buffer
- PDH Substrate
- PDH Developer
- NADH Standard
- PDH Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
存储类别
8A - Combustible corrosive hazardous materials
法规信息
低风险生物材料
此项目有
Minfeng Ying et al.
The Journal of biological chemistry, 294(24), 9615-9630 (2019-05-02)
Whether growing cancer cells prefer lactate as a fuel over glucose or vice versa is an important but controversial issue. Labeling of tricarboxylic acid (TCA) cycle intermediates with glucose or lactate isotope tracers is often used to report the relative
The Citric Acid Cycle Oxidizes Two-Carbon Units
Berg J M, et al.
Biochemistry (2002)
Ryan M Bradley et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1863(7), 700-711 (2018-04-09)
Lysophosphatidic acid acyltransferase (LPAAT) δ/acylglycerophosphate acyltransferase 4 is a mitochondrial enzyme and one of five homologues that catalyze the acyl-CoA-dependent synthesis of phosphatidic acid (PA) from lysophosphatidic acid. We studied skeletal muscle LPAATδ and found highest levels in soleus, a
Pyruvate as a pivot point for oncogene-induced senescence.
Olenchock B A and Matthew G V H
Cell, 153(7), 1429-1430 (2013)
The spectrum of pyruvate dehydrogenase complex deficiency: clinical, biochemical and genetic features in 371 patients.
Patel K P, et al.
Molecular Genetics and Metabolism, 106(3), 385-394 (2012)
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持