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Merck
CN

MAK193

柠檬酸合成酶活性检测试剂盒

Sufficient for 100 Colorimetric tests

别名:

柠檬酸合成酶检测试剂盒

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关于此项目

UNSPSC Code:
12161503
EC Number:
232-821-7
NACRES:
NA.84
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产品名称

柠檬酸合成酶活性检测试剂盒, Sufficient for 100 Colorimetric tests

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

Gene Information

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

Application

柠檬酸合酶活性检测试剂盒已用于测量样品中柠檬酸合酶的活性。
适于测定不同生物样品内的柠檬酸合成酶活性,如细胞和组织培养上清液以及纯化所得的线粒体。

Biochem/physiol Actions

柠檬酸合酶活性检测试剂盒提供了一种简单直接的方法,用于测量各种组织、细胞培养物(粘附或悬浮)和纯化线粒体中柠檬酸合酶的活性。使用偶联酶反应测定柠檬酸合酶活性,其产生与存在的酶活性成比例的比色(412 nm)产物。一个单位的柠檬酸合酶是在25 °C和pH 7.2 下,每分钟产生 1.0 mmole CoA的酶的量。

General description

柠檬酸合酶(CS;EC 2.3.3.1)是一种线粒体酶,存在于所有能够进行氧化代谢的细胞中。它在Krebs 三羧酸循环中,催化草酰乙酸合成柠檬酸盐。1,2柠檬酸合酶参与能量产生、脂肪生成和胆固醇生成,其活性遵循昼夜节律模式。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

存储类别

10 - Combustible liquids

wgk

WGK 3

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

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Moritz A Niederschweiberer et al.
Frontiers in molecular biosciences, 8, 671274-671274 (2021-07-02)
Alzheimer's disease (AD), the most prevalent form of dementia, affects globally more than 30 million people suffering from cognitive deficits and neuropsychiatric symptoms. Substantial evidence for the involvement of mitochondrial dysfunction in the development and/or progression of AD has been shown
P Dongil et al.
Scientific reports, 8(1), 13810-13810 (2018-09-16)
Metabolic dysfunction in the liver is the cause of numerous pathologies, which are associated with an altered redox state. PASK (PAS Domain Kinase) is a nutrient and bioenergetic sensor. We contend that PASK could act as an oxidative stress sensor
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)
Tomoaki Aoki et al.
Frontiers in medicine, 8, 666735-666735 (2021-04-30)
Background: Identification of the mechanisms underlying mitochondrial dysfunction is key to understanding the pathophysiology of acute injuries such as cardiac arrest (CA); however, effective methods for measurement of mitochondrial function associated with mitochondrial isolation have been debated for a long
Tony Chao et al.
American journal of physiology. Cell physiology, 317(6), C1229-C1238 (2019-09-19)
Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte

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