detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
Gene Information
human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)
General description
柠檬酸合酶（CS；EC 2.3.3.1）是一种线粒体酶，存在于所有能够进行氧化代谢的细胞中。它在Krebs 三羧酸循环中，催化草酰乙酸合成柠檬酸盐。1,2柠檬酸合酶参与能量产生、脂肪生成和胆固醇生成，其活性遵循昼夜节律模式。
Application
柠檬酸合酶活性检测试剂盒已用于测量样品中柠檬酸合酶的活性。
适于测定不同生物样品内的柠檬酸合成酶活性，如细胞和组织培养上清液以及纯化所得的线粒体。
Biochem/physiol Actions
柠檬酸合酶活性检测试剂盒提供了一种简单直接的方法，用于测量各种组织、细胞培养物（粘附或悬浮）和纯化线粒体中柠檬酸合酶的活性。使用偶联酶反应测定柠檬酸合酶活性，其产生与存在的酶活性成比例的比色（412 nm）产物。一个单位的柠檬酸合酶是在25 °C和pH 7.2 下，每分钟产生 1.0 mmole CoA的酶的量。
仅试剂盒组分
产品编号
说明
- CS Assay Buffer
- CS Substrate Mix
- CS Developer
- GSH Standard, Reduced
- CS Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 3
法规信息
低风险生物材料
此项目有
Pilar Dongil et al.
Aging, 12(3), 2275-2301 (2020-01-25)
Several signaling pathways may be affected during aging. All are regulated by nutrient levels leading to a decline in mitochondrial function and autophagy and to an increase in oxidative stress. PAS Domain Kinase (PASK) is a nutrient and bioenergetic sensor.
Xi Lin et al.
International journal of molecular sciences, 21(3) (2020-01-26)
Maintaining an active fatty acid metabolism is important for renal growth, development, and health. We evaluated the effects of anaplerotic and ketogenic energy sources on fatty acid oxidation during stimulation with clofibrate, a pharmacologic peroxisome proliferator-activated receptor α (PPARα) agonist.
Md Habibur Rahman et al.
Microsystems & nanoengineering, 4, 39-39 (2019-05-07)
Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders, cancers and cardiovascular disease. However, the detailed characterization of mitochondria has remained relatively unexplored, largely due to the lack of effective extraction methods that may sufficiently
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)
P Dongil et al.
Scientific reports, 8(1), 13810-13810 (2018-09-16)
Metabolic dysfunction in the liver is the cause of numerous pathologies, which are associated with an altered redox state. PASK (PAS Domain Kinase) is a nutrient and bioenergetic sensor. We contend that PASK could act as an oxidative stress sensor
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