Cathepsin B Activity Assay Kit

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

The kit is suitable for the detection of Cathepsin-B activity in cell lysates.

The Cathepsin-B Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorescence plate reader.

The Cathepsin-B Activity Assay kit:

• Is a simple one-step procedure with results in 1-2 hours.