谷胱甘肽 GSH/GSSG 检测试剂盒

谷胱甘肽是甘氨酸、谷氨酸和半胱氨酸的三肽，它是保护细胞免受活性氧 导致的损伤的关键抗氧化剂之一。谷胱甘肽 （GSH）将细胞质蛋白中的二硫键还原为半胱氨酸，在此过程中中它被转化为其氧化形式 GSSG。

Glutathione (GSH), composed of the amino acids glycine, glutamic acid, and cysteine, serves as a fundamental antioxidant within the body, playing a pivotal role in cellular defense against oxidative stress caused by reactive oxygen species (ROS). As the major free thiol in cells, GSH is instrumental in various biological processes, including the detoxification of harmful substances, the removal of hydroperoxides, and the maintenance of protein sulfhydryl groups in their reduced state. GSH achieves this by directly reducing disulfide bonds in cytoplasmic proteins to cysteines, during which it is transformed into its oxidized form, glutathione disulfide (GSSG). This cycle between reduced and oxidized forms is critical for maintaining the cellular redox balance.Glutathione functions as a cofactor for several key antioxidant enzymes, enhancing their ability to neutralize free radicals. It also plays a crucial role in the regeneration of vitamins C and E, thereby contributing to their antioxidative effects. Beyond its antioxidant properties, GSH is vital for mitochondrial function and the integrity of mitochondrial DNA (mtDNA), which is essential for energy production and cellular health. Dysregulation of glutathione levels is associated with the pathogenesis of several neurodegenerative diseases such as Alzheimer′s, Parkinson′s, and Huntington′s diseases, as well as other health conditions including cystic fibrosis, immune disorders, and cardiovascular diseases. Glutathione′s role in metabolism and cellular protection is extensive, influencing cellular proliferation, apoptosis, and overall physiological stability.

The Glutathione GSH/GSSG Assay Kit may be used for:

• Cancer Research
• Diabetes Research
• Neurodegenerative Disease Research

Sensitive Detection Range: Detect Glutathione levels accurately across a wide range, from 0.01 µM to 3 µM, using only 25 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 10 minute room temperature reaction, reducing complexity and saving valuable time and effort.Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

该试剂盒适用于全血、血浆、血清、尿液、组织和细胞提取物中还原型和氧化型谷胱甘肽（GSH/GSSG）的定量测定。

谷胱甘肽 GSH/GSSG 检测试剂盒设计用于采用埃尔曼试剂 (DTNB) 和谷胱甘肽还原酶 (GR) 以酶法准确测定生物样品中的总谷胱甘肽、还原型谷胱甘肽和氧化型谷胱甘肽。DTNB 与还原型谷胱甘肽反应形成一种黄色产物。在 ₄₁₂nm 处测量的光密度变化率与样品中的谷胱甘肽浓度成正比。该试剂盒还可用于以一个特定实验方案测定氧化型 GSSG，该方案首先以 1-甲基-2-乙烯基吡啶鎓三氟甲磺酸盐清除所有 GSH 。

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