产品名称
L-Lactate Assay Kit, Sufficient for 100 fluorometric tests
input
cell culture medium
plasma
serum
urine
detection method
fluorometric
relevant disease(s)
diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer
storage temp.
−20°C
Quality Level
Application
The L-Lactate Assay Kit may be used for:
- Lactate Metabolism Research
- Tissue Metabolism Research
- Drug Discovery
The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.
Biochem/physiol Actions
The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.
Features and Benefits
- Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
- Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
- High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.
General description
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
存储类别
12 - Non Combustible Liquids
法规信息
含少量动物源组分生物产品
常规特殊物品
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Instructions
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