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Merck
CN

MAK450

Sigma-Aldrich

Glucose Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

别名:

GDH Activity Assay Kit

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关于此项目

UNSPSC代码:
12161503
NACRES:
NA.84
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质量水平

储存条件

(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)

输入

tissue
cell culture medium
tissue
plasma

检测方法

colorimetric

相关疾病

endocrinological disorders, diabetes; hematological disorder

储存温度

−20°C

一般描述

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

应用

The Glucose Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

生化/生理作用

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

特点和优势

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

其他说明

For additional information on our range of Biochemicals, please complete this form.

警示用语:

Danger

危险分类

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

3 - Flammable liquids

法规信息

常规特殊物品
含少量动物源组分生物产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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