Glucose Dehydrogenase Assay Kit

Name: Glucose Dehydrogenase Assay Kit
Brand: SIGMA

Sufficient for 100 colorimetric tests

别名:

GDH Activity Assay Kit

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关于此项目

NACRES:

NA.84

UNSPSC Code:

12161503

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属性

Quality Level

100

storage condition

(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)

input

cell culture medium
tissue
plasma

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; hematological disorder

storage temp.

−20°C

说明

General description

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

Application

The Glucose Dehydrogenase Assay Kit may be used for:

Metabolism Research
Drug Discovery

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

Biochem/physiol Actions

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Features and Benefits

Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.