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Merck
CN

MAK525

Sigma-Aldrich

Acid Phosphatase Fluorometric Assay Kit

sufficient for 100 fluorometric tests

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关于此项目

UNSPSC代码:
12161503
NACRES:
NA.84
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质量水平

检测方法

fluorometric

相关疾病

genitourinary disorders; cancer; gastrointestinal diseases

运输

wet ice

储存温度

−20°C

一般描述

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

应用

The kit is suitable for acid phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples).

生化/生理作用

The Acid Phosphatase Fluorometric Assay Kit is based on the cleavage of a synthetic substrate. The product methylumbelliferone (MUB) becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at λEx = 360 nm/λEm = 450 nm after addition of the stop reagent is directly proportional to the enzyme activity.

特点和优势

  • Linear detection range: 0.008 to 10 U/L .
  • Compatible with high-throughput handling systems.

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 1

法规信息

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Lot/Batch Number

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