LDH Cytotoxicity Assay Kit

Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme, that upon membrane damage, is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage.

The kit is suitable for the rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium, and for the evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.

The LDH Cytotoxicity Assay Kit is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.