Butyrylcholinesterase (BChE) Activity Assay Kit

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized in liver and present in blood. BChE is a nonspecific cholinesterase enzyme and can hydrolyze many different choline esters, serving as the first line of defense against toxic compounds reaching the bloodstream. ​

The Butyrylcholinesterase Activity Assay Kit is based on a synthetic butyrylthiocholine based substrate, which can be hydrolyzed by BChE and produce thiocholine. Thiocholine can react with 5, 5′-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be detected at 410 nm. The assay is convenient, sensitive and can detect as low as 6 mU/mL in a variety of samples.