Peroxide Assay Kit

Peroxide (hydrogen peroxide H₂O₂) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as aging, asthma, diabetes, atherosclerosis, cataracts, inflammatory arthritis and neurodegenerative diseases.

Suitable for the direct assay of H₂O₂ and organic peroxides in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium) and the determination of the effects of drugs on peroxide metabolism.

The improved peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. This method utilizes the chromogenic Fe³⁺‑xylenolorange reaction, in which a purple complex is formed when Fe²⁺provided in the reagent is oxidized to Fe³⁺ by peroxides present in the sample. The intensity of the color, measured at 540‑610 nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

This assay has a detection limit of 0.54 µM (18.4 ng/mL) and linear up to 30 µM (1,020 ng/mL) H₂O₂ in a 96-well plate assay.