Merck
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MCL1

Sigma-Aldrich

哺乳动物细胞裂解试剂盒

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NACRES:
NA.56

用途

 kit sufficient for 250 extractions (100 mm tissue culture plates)

质量水平

运输

dry ice

储存温度

−20°C

应用

本产品含有分解哺乳动物细胞和组织以便于之后分析蛋白目标基因所需的一切试剂。在裂解过程中,胞内蛋白保持稳定,适于进行免疫检测、免疫沉淀、亲和纯化及其他功能检测。
哺乳动物细胞裂解试剂盒以用于检测血管内皮生长因子(VEGF) 受体-1及其配体VEGF-B在活动神经元退化过程中发挥的作用。

特点和优势

  • 该试剂盒可用于制备细胞裂解缓冲液和其他改性缓冲液。
  • 该试剂盒允许研究人员调整缓冲液成分,从而获得对于特定蛋白质最高效的缓冲液。

组分

哺乳动物细胞理解试剂盒包含各种可以混合的溶液,可用于裂解贴壁、未贴壁的细胞及组织。其含有缓冲液、去垢剂、Nacl、蛋白质抑制物混合溶液。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • P8340Protease inhibitor cocktail 2.5 mL化学品安全说明书

象形图

CorrosionExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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T1503
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Shintaro Nakao et al.
Investigative ophthalmology & visual science, 54(6), 3830-3836 (2013-04-13)
Inflammation and immune cells regulate choroidal neovascularization (CNV) and could become therapeutic targets in age-related macular degeneration (AMD). Lymphangiogenesis is a key component of various inflammatory diseases. Whether lymphangiogenesis and lymph node-mediated immunity are involved in the pathogenesis of AMD
Shinsuke Yasuda et al.
Journal of immunology (Baltimore, Md. : 1950), 179(7), 4890-4900 (2007-09-20)
Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from
Shintaro Nakao et al.
Blood, 117(3), 1081-1090 (2010-08-14)
Angio- and lymphangiogenesis are inherently related processes. However, how blood and lymphatic vessels regulate each other is unknown. This work introduces a novel mechanism explaining the temporal and spatial relation of blood and lymphatic vessels. Vascular endothelial growth factor-A (VEGF-A)
Shintaro Nakao et al.
The American journal of pathology, 178(4), 1913-1921 (2011-03-26)
Vascular adhesion protein-1 (VAP-1) contributes to inflammatory and angiogenic diseases, including cancer and age-related macular degeneration. It is expressed in blood vessels and contributes to inflammatory leukocyte recruitment. The cytokines IL-1β and vascular endothelial growth factor A (VEGF-A) modulate angiogenesis
Lech Martyniszyn et al.
Viral immunology, 26(5), 322-335 (2013-10-15)
Several studies have provided evidence that complex relationships between autophagic and apoptotic cell death pathways occur in cancer and virus-infected cells. Previously, we demonstrated that infection of macrophages with Moscow strain of ectromelia virus (ECTV-MOS) induces apoptosis under in vitro

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