usage
kit sufficient for 250 extractions (100 mm tissue culture plates)
technique(s)
immunoprecipitation (IP): suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
本产品含有分解哺乳动物细胞和组织以便于之后分析蛋白目标基因所需的一切试剂。在裂解过程中，胞内蛋白保持稳定，适于进行免疫检测、免疫沉淀、亲和纯化及其他功能检测。
哺乳动物细胞裂解试剂盒以用于检测血管内皮生长因子(VEGF) 受体-1及其配体VEGF-B在活动神经元退化过程中发挥的作用。
哺乳动物细胞裂解试剂盒以用于检测血管内皮生长因子(VEGF) 受体-1及其配体VEGF-B在活动神经元退化过程中发挥的作用。
Features and Benefits
- 该试剂盒可用于制备细胞裂解缓冲液和其他改性缓冲液。
- 该试剂盒允许研究人员调整缓冲液成分，从而获得对于特定蛋白质最高效的缓冲液。
Other Notes
哺乳动物细胞理解试剂盒包含各种可以混合的溶液，可用于裂解贴壁、未贴壁的细胞及组织。其含有缓冲液、去垢剂、Nacl、蛋白质抑制物混合溶液。
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Shintaro Nakao et al.
Blood, 117(3), 1081-1090 (2010-08-14)
Angio- and lymphangiogenesis are inherently related processes. However, how blood and lymphatic vessels regulate each other is unknown. This work introduces a novel mechanism explaining the temporal and spatial relation of blood and lymphatic vessels. Vascular endothelial growth factor-A (VEGF-A)
Shintaro Nakao et al.
Investigative ophthalmology & visual science, 54(6), 3830-3836 (2013-04-13)
Inflammation and immune cells regulate choroidal neovascularization (CNV) and could become therapeutic targets in age-related macular degeneration (AMD). Lymphangiogenesis is a key component of various inflammatory diseases. Whether lymphangiogenesis and lymph node-mediated immunity are involved in the pathogenesis of AMD
Shinsuke Yasuda et al.
Journal of immunology (Baltimore, Md. : 1950), 179(7), 4890-4900 (2007-09-20)
Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from
Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: a novel biomarker for early diagnosis.
Sun D, Nakao S, Xie F, et al.
Faseb Journal (2014)
Jie Liu et al.
Frontiers in physiology, 6, 69-69 (2015-03-26)
In many non-excitable and excitable cells, store-operated calcium entry (SOCE) represents an additional pathway for calcium entry upon Ca(2+) store depletion. In a previous study, we demonstrated SOCE activity in intact mouse cardiac pacemaker tissue, specifically from sinoatrial node (SAN)
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