packaging
pkg of 1 kit
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable
fluorescence
λex 490 nm; λem 502 nm (PKH67 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
Quality Level
General description
此试剂盒用于常规细胞膜标记。PKH67具有比PKH1和PKH2（之前在体外和体内细胞示踪应用体积的另外两种绿色染料）更长的脂肪族碳尾。由于具有更长的尾部，内部研究一致表明与PKH2相比，PKH67的细胞转移减少。
使用PKH1和PKH2进行的体内研究观察到荧光缓慢消失。PKH67可能表现出相似性质，因为这种行为似乎是绿色细胞接头染料的特征，而非红色细胞接头染料的特征。 非分裂期细胞的体外细胞膜的保留性质与体内荧光半衰期之间的相关性，预测PKH67的体内荧光半衰期为10-12天。 其他半衰期相似的绿色细胞接头染料已用于监测1-2个月内体内淋巴细胞和巨噬细胞运输，表明PKH67还可用于中等长度的体内示踪研究。
使用PKH1和PKH2进行的体内研究观察到荧光缓慢消失。PKH67可能表现出相似性质，因为这种行为似乎是绿色细胞接头染料的特征，而非红色细胞接头染料的特征。 非分裂期细胞的体外细胞膜的保留性质与体内荧光半衰期之间的相关性，预测PKH67的体内荧光半衰期为10-12天。 其他半衰期相似的绿色细胞接头染料已用于监测1-2个月内体内淋巴细胞和巨噬细胞运输，表明PKH67还可用于中等长度的体内示踪研究。
Application
PKH67绿色荧光细胞接头试剂盒(Midi)已用于：
- 标记外泌小体
- 胞外囊泡(EV)l
- 正常和镰状红细胞 (RBCs)
- Raji cells细胞
Other Notes
如需关于PKH及CellVue®的荧光细胞连接子染料的更多技术详情，包括大量的参考书目，请访问这里。
Legal Information
CellVue is a registered trademark of Phanos Technologies
仅试剂盒组分
产品编号
说明
- PKH67 Dye 2 x 0.1
- Diluent C 6 x 10
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
存储类别
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
法规信息
危险化学品
此项目有
Dongxin Tang et al.
Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The
Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Bach F, et al.
Testing, 8(51), 88845-88845 (2017)
Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
Alshaiban A, et al.
Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
ADCC Assay Protocol
Fang L and He F
Journal of Virology (2013)
Chuang Gao et al.
Aging, 11(24), 12147-12164 (2019-12-17)
Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The
商品
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
最佳的染色结果是研究肿瘤发生和进展的一项关键组成部分。了解关于染色应用的有用建议和技术，包括来自近期研究的案例。
PKH和CellVue® 荧光细胞连接子试剂盒可提供长时间的活细胞荧光标记，且无明显毒性作用。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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