MITOISO3
酵母线粒体分离试剂盒
sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells
产品名称
酵母线粒体分离试剂盒, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells
usage
sufficient for 40 applications (using 20 OD culture preparations)
storage temp.
−20°C
Quality Level
Application
该试剂盒包含通过溶壁酶裂解酵母细胞壁（原生质球形成），然后进行细胞膜裂解/破碎和线粒体分离所需的所有试剂。另外，该试剂盒包括用于蛋白质组学研究的线粒体蛋白质谱分析提取缓冲液和用于完整线粒体的存储缓冲液。酵母线粒体分离试剂盒已被用于线粒体的分离和提取。
Features and Benefits
• 该试剂盒具有快速、灵敏、易用、特异性强的特征
• 该试剂盒含有通过溶细胞酶(lyticase)（微球形式）轻松、快速地溶解细胞壁，以及之后的细胞膜裂解/破坏及线粒体分离所需的一切试剂。
• 该试剂盒为细胞膜破碎提供了两种替代方法。 第一种方法是降解，第二种方法是均质化。
• 该试剂盒包含一份可用于线粒体蛋白谱分析的提取缓冲液，可供蛋白质组研究使用
• 该试剂盒包含用于完整线粒体的储液。
• 所含的试剂足够进行40次20 OD培养物的处理
• 该试剂盒已在Saccharomyces cerevisiae、Pichia pastoris和Schizosaccharomyces pombe上进行过测试
• 该试剂盒含有通过溶细胞酶(lyticase)（微球形式）轻松、快速地溶解细胞壁，以及之后的细胞膜裂解/破坏及线粒体分离所需的一切试剂。
• 该试剂盒为细胞膜破碎提供了两种替代方法。 第一种方法是降解，第二种方法是均质化。
• 该试剂盒包含一份可用于线粒体蛋白谱分析的提取缓冲液，可供蛋白质组研究使用
• 该试剂盒包含用于完整线粒体的储液。
• 所含的试剂足够进行40次20 OD培养物的处理
• 该试剂盒已在Saccharomyces cerevisiae、Pichia pastoris和Schizosaccharomyces pombe上进行过测试
General description
作为真核细胞能产生能量的地点，线粒体的特征是双层膜结构：外膜和折叠的内膜。分离所得的线粒体可用作进行体外研究的工具，如呼吸和能量生成研究，凋亡、mtDNA和mtRNA、以及线粒体蛋白谱分析。
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 2
存储类别
10 - Combustible liquids
法规信息
监管及禁止进口产品
此项目有
Yao Wan et al.
Genes & development, 32(19-20), 1309-1314 (2018-09-20)
The mitochondrial cytoplasmic surface serves as a processing site for numerous RNAs from budding yeast to metazoans. We report that budding yeast mitochondrial outer membrane (MOM) proteins that are subunits of the translocase of the outer mitochondrial membrane (Tom70 and
Maria Guirola et al.
Metallomics : integrated biometal science, 6(3), 634-645 (2014-02-08)
Zinc is an essential metal for all organisms, as it participates in the structure and/or function of many proteins. However, zinc excess is as deleterious to cells as zinc deficiency. A genome-wide study of the transcriptomic response to high zinc
Panos Oikonomou et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(43), 26710-26718 (2020-10-11)
Large-scale proteomic methods are essential for the functional characterization of proteins in their native cellular context. However, proteomics has lagged far behind genomic approaches in scalability, standardization, and cost. Here, we introduce in vivo mRNA display, a technology that converts
María Guirola et al.
The Biochemical journal, 432(3), 595-605 (2010-09-23)
The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear DNA integrity. Our previous work revealed that a pif1Δ strain is tolerant to zinc overload. In the present study we demonstrate that this
Yeast cells expressing the human mitochondrial DNA polymerase reveal correlations between polymerase fidelity and human disease progression
Qian Y, et al.
The Journal of Biological Chemistry (2017)
商品
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
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