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Merck
CN

MP0025

Venor GeM 支原体检测试剂盒,基于 PCR

别名:

PCR mycoplasma detection kit

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
Application(s):
detection
microbiology
Technique(s):
PCR: suitable
Storage temp.:
2-8°C
技术服务
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usage

 kit sufficient for 25 tests

Quality Level

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

General description

Venor GeM 支原体检测试剂盒利用聚合酶链反应(PCR),成为检测细胞培养物和其它细胞培养衍生生物制品中支原体无胆甾原体污染的灵敏度最高的首选方法。检测需要低至 1-5fg 的支原体 DNA,对应于每个样品 2-5 个支原体。引物组对高度保守的 rRNA 操纵子有特异性,更具体而言,对支原体基因组中的 16S rRNA 编码区有特异性。这让我们可以检测迄今为止测试的所有支原体无胆甾原体脲原体物种,这些物种通常是细胞培养物中的污染物。该试剂盒不可扩增真核和细菌 DNA。

Application

基于 PCR 的 Venor GeM 支原体检测试剂盒采用 PCR 技术快速可靠地检测细胞培养物和病毒原液中的支原体 DNA。

Preparation Note

该试剂盒在环境温度下运输时是稳定的。收货后,在 2–8 °C下储存。 引物/核苷酸混合物、阳性对照和内对照再水化后,在 –20 °C 以下储存,避免反复冻融。对于低样品数的重复测试,引物/核苷酸混合物和对照应在再水化后等分。遵循这些建议,该试剂盒在标签上注明的失效日期之前是稳定的。

Other Notes

不包括Taq聚合酶。 优化用于 D9307、Taq DNA 聚合酶

Legal Information

Venor is a trademark of Minerva Biolabs GmbH

仅试剂盒组分

产品编号
说明

  • Positive Control

  • Negative Control

  • PCR 10X Reaction Buffer 500 mL/vial

  • Primer/Nucleotide Mix 1 mL/vial

存储类别

10 - Combustible liquids

wgk

WGK 3

法规信息

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Julie V Philley et al.
Journal of cellular physiology, 231(6), 1364-1374 (2015-11-05)
Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa
Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management
Russell BJ, et al.
Journal of Virological Methods (2020)
Yanyan Li et al.
Oncotarget, 8(63), 106311-106323 (2018-01-02)
Decades of cytotoxic and more recently immunotherapy treatments for malignant glioma have had limited success due to dynamic intra-tumoral heterogeneity. The dynamic interplay of cancer cell subpopulations has been found to be under the control of proteins in the cancer
Enhanced neonatal Fc receptor function improves protection against primate SHIV infection.
Ko SY, Pegu A, Rudicell RS, et al.
Nature, 514(7524), 642-645 (2014)
Anbarasu Kannan et al.
Scientific reports, 9(1), 11632-11632 (2019-08-14)
Triple negative breast cancer (TNBC) has poor survival, exhibits rapid metastases, lacks targeted therapies and reliable prognostic markers. Here, we examined metastasis promoting role of cancer testis antigen SPANXB1 in TNBC and its utility as a therapeutic target and prognostic

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实验方案

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

全球贸易项目编号

货号GTIN
MP0025-1KT04061834066811

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