Merck
CN

MS0100

Supelco

ProteoMass Guanidination Kit

For improving MALDI-MS sensitivity and peptide sequence coverage

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NACRES:
NA.24

产品线

ProteoMass

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

相容性

for use with (Complex cell extracts, pure protein solution, 1D and 2D PAGE gels)

应用

ProteoMass Guanidination Kit 有助于增强MALDI-MS 的灵敏性,提高序列覆盖度,更可靠地对多肽进行鉴定。蛋白水解消化后,C端带有精氨酸残基的多肽相比C端带赖氨酸残基的多肽会更优先离子化,从而使得多肽质谱分析中序列覆盖度受影响,可靠度有限。ProteoMass Guanidination Kit 可以高效方便地将C端赖氨酸残基转化为高精氨酸,提高MALDI的信号强度,并获得更好的序列覆盖度。

特点和优势

  • 更精确可靠地鉴定更多的样品
  • 提高通量,节省时间—使用试剂盒仅需35分钟 vs. 传统方法需要2小时
  • 兼容性—可用于1D或2D的PAGE凝胶条带或点,也可用于复杂的细胞提取物。

其他说明

一盒MS0100 试剂盒可进行96次反应。

法律信息

ProteoMass is a trademark of Sigma-Aldrich Co. LLC

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Roberta Cozzi et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(5), 2008-2018 (2012-01-19)
Group B Streptococcus pili are covalently linked structures assembled via a sortase-catalyzed transpeptidation mechanism involving specific residues and motifs. A sequence element containing a conserved glutamic acid, called the E-box, has been described to be involved in pilus formation. Although
Alberto Nuñez et al.
Journal of agricultural and food chemistry, 57(22), 10951-10958 (2009-10-29)
Several studies have suggested that the emulsification properties associated with pectin obtained from sugar beet (Beta vulgaris) are due to the presence of a protein-pectin complex. Nevertheless, the identity of the protein has remained elusive. Pectin, extracted from sugar beet
Fumihiro Oshita et al.
Oncology reports, 24(3), 637-645 (2010-07-29)
To investigate the early changes in protein function that induce micro-metastasis in early-stage non-small cell lung cancer, we conducted proteomic analysis of tissue that had been completely resected. We selected sixteen patients whose tumors were pathological stage I adenocarcinoma with
Yoshimi Kanie et al.
PloS one, 4(5), e5434-e5434 (2009-05-06)
A variety of N-glycans attached to protein are known to involve in many important biological functions. Endoplasmic reticulum (ER) and Golgi localized enzymes are responsible to this template-independent glycan synthesis resulting glycoforms at each asparagine residues. The regulation mechanism such
Nicolas Vogel et al.
The Journal of biological chemistry, 284(52), 36128-36136 (2009-10-29)
The strychnine-sensitive glycine receptor (GlyR) is a ligand-gated chloride channel and a member of the superfamily of cysteine loop (Cys-loop) neurotransmitter receptors, which also comprises the nicotinic acetylcholine receptor (nAChR). Within the extracellular domain (ECD), the eponymous Cys-loop harbors two

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