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MSQC4

SILu™Lite SigmaMAb 通用抗体标准品 人

别名:

IgG1 光, 人 SILu™Lite SigmaMAb 通用抗体标准品, SigmaMAb

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UNSPSC代码:
23201100
NACRES:
NA.12

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重组

expressed in CHO cells

质量水平

200

运输

wet ice

储存温度

−20°C

相关类别

一般描述

SILU™ Lite SigmaMAb 是一种重组人单克隆 IgG1λ 轻抗体，在 CHO 细胞中表达的分子量约为 150 kDa。其旨在优化单克隆抗体、生物仿制药和药品的准确完整质量分析。对这种大型生物分子进行准确的完整质量分析可以提供有关结构和翻译后修饰（如糖基化）的全面信息。其他信息如异质性、批间变异、氨基酸截断以及 N-末端 Lys 加工、聚集和降解等均可确定。在治疗性单克隆抗体药物开发中，使用质谱法进行完整质量分析对于配制和储存也非常重要。
该产品由两条相同的重链和两条相同的轻链组成。重链和轻链通过一个二硫键连接。重链通过位于铰链区的两个二硫键连接。其他 12 个半胱氨酸键在分子内仅限于 6 个不同的球状结构域。采用 4 种不同的酶：糜蛋白酶、Asp-N 和 Glu-C 内切蛋白酶和胰蛋白酶，通过完整质量和肽图谱分析评价抗体序列。获得了 100% 的序列覆盖率。

应用

以 SILu™ Lite SigmaMAb 通用抗体标准人为模型系统，研究气相单克隆抗体 (mAb) 解折叠与 mAb 糖基化离散水平之间的定量关系。

特点和优势

SigmaMAb 轻链、重链和完整蛋白（糖型丰度最高）的分子量计算值如下：

描述/组成/更改/平均质量 (Da)

还原轻链/C1006H1555N267O333S7 / 焦谷氨酸 (Q) / 22942.2

还原重链/C2181H3393N587O663S16 / （无更改）/ 48957.8
C2237H3485N591O702S16 / G0F / 50403.2
C2243H3495N591O707S16 / G1F / 50565.3
C2249H3505N591O712S16 / G2F / 50727.5

天然完整质量，非还原型 / C6374H9864N1708O1992S46 / 2 X 焦谷氨酸 (Q) / 143767.7
C6486H10048N1716O2070S46 / G0F+G0F / 146658.4
C6492H10058N1716O2075S46 / G0F+G1F / 146820.6
C6498H10068N1716O2080S46 / G1F+G1F / 146982.7
C6504H10078N1716O2085S46 / G1F+G2F / 147144.8
C6510H10088N1716O2090S46 / G2F+G2F / 147307.0

外形

以含有磷酸盐缓冲盐水的冻干粉形式提供

制备说明

当使用磷酸盐缓冲液 (pH 值为 6-7) 复溶冻干品时，SigmaMAb 回收率最大。

分析说明

SigmaMab 重链
EVQLVESGGGLVQPGGSLRLSCVASGFTLNNYDMHWVRQGIGKGLEWVSKI
GTAGDRYYAGSVKGRFTISRENAKDSLYLQMNSLRVGDAAVYYCARGAGRW
APLGAFDIWGQGTMVTVSS|ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYF
PEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVN
HKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR
TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRV
VSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPS
RDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFL
YSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG

SigmaMab 轻链
QSALTQPRSVSGSPGQSVTISCTGTSSDIGGYNFVSWYQQHPGKAPKLMIY
DATKRPSGVPDRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGDYTPGV
VFGGGTKLTVL|GQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTV
AWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQ
VTHEGSTVEKTVAPTECS
也可作为稳定同位素标记产品，Silu ™Mab（产品编号 MSQC3）
根据 A280 测定的蛋白含量确定包装规格

其他说明

避免使用 PBS 进行复溶。
通过加入 500 μL 超纯水或磷酸盐缓冲液复溶小瓶内容物，并剧烈混匀。溶解后的产物可根据需要进一步稀释。

法律信息

SILu is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000475872
0000475872
0000423354
0000423354
0000407636

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Jared B Shaw et al.
Analytical chemistry, 90(18), 10819-10827 (2018-08-18)
Compared to traditional collision induced dissociation methods, electron capture dissociation (ECD) provides more comprehensive characterization of large peptides and proteins as well as preserves labile post-translational modifications. However, ECD experiments are generally restricted to the high magnetic fields of FTICR-MS
Daniel A Polasky et al.
Analytical chemistry, 91(4), 3147-3155 (2019-01-23)
Ion mobility-mass spectrometry (IM-MS) has become an important addition to the structural biology toolbox, but separating closely related protein conformations remain challenging. Collision-induced unfolding (CIU) has emerged as a valuable technique for distinguishing iso-cross-sectional protein and protein complex ions through
Yutong Jin et al.
mAbs, 11(1), 106-115 (2018-09-20)
The pharmaceutical industry's interest in monoclonal antibodies (mAbs) and their derivatives has spurred rapid growth in the commercial and clinical pipeline of these effective therapeutics. The complex micro-heterogeneity of mAbs requires in-depth structural characterization for critical quality attribute assessment and
Anna C Robotham et al.
mAbs, 11(4), 757-766 (2019-03-22)
The detection of free sulfhydryls in proteins can reveal incomplete disulfide bond formation, indicate cysteine residues available for conjugation, and offer insights into protein stability and structure. Traditional spectroscopic methods of free sulfhydryl detection, such as Ellman's reagent, generally require
"Collision Induced Unfolding Detects Subtle Differences in Intact Antibody Glycoforms and Associated Fragments.
Tian Y and Brandon T R
International Journal of Mass Spectrometry (2017)
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实验方案

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