MTOX1105
MRP3 Knockout HepaRG™ Cells
human female liver (hepatocarcinoma and hepatitis C)
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关于此项目
UNSPSC Code:
12352207
NACRES:
NA.81
eCl@ss:
32150313
Biological source:
human female liver (hepatocarcinoma and hepatitis C)
产品名称
MRP3 Knockout HepaRG™ Cells, 1 vial
biological source
human female liver (hepatocarcinoma and hepatitis C)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... MRP3(8714)
General description
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MRP3 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
Application
See technical bulletin for detailed protocols
Features and Benefits
HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.
- Sigma′s HepaRG MRP3 Knockout (KO) allows investigations of drug-transporter interactions involving MRP3 in the liver.
Zinc finger nucleases (ZFN) mediated Knock-out of ABCC3 (MRP3) gene.
- The frame-shift mutation of ABCC3 gene was confirmed by fragment length analysis and DNA sequencing.
- Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
Legal Information
These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
存储类别
12 - Non Combustible Liquids
WGK
WGK 3
闪点 (F)
Not applicable
闪点 (°C)
Not applicable