sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 70 mL of HepaRG™ Maintenance Medium Supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of HepaRG™ cell line. The final HepaRG maintenance medium was developed specifically for use with Sigma′s human HepaRG™ control 5F Clone cells and various knockouts (KO).
Preparation Note
Prepare Maintenance Medium by supplementing 500 mL of William′s E Medium (W1878) ) with 5 mL of Penicillin-Streptomycin solution (P4333), 5 mL of GlutaMAX (Life Technologies 35050-061), and the entire Maintenance Medium Supplement (MTOXHMSUP). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.
Other Notes
Upon receiving a shipment of supplement(s) it is important the end user gives the shipment attention without delay. To ensure the highest quality of the supplement(s), upon receipt supplements should be immediately stored at –20 °C. Upon thaw, medium supplement will be clear to turbid. Upon addition to the Williams′ E Medium, medium should be clear.
Legal Information
HepaRG is a trademark of BioPredic International company
signalword
Warning
hcodes
Hazard Classifications
Repr. 2
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable