General description
凝胶过滤色谱是一种广泛使用的检测蛋白质大小和分子量的方法。蛋白质大小的差别使其进入树脂孔穴内的程度也不同，从而实现分离。较大的蛋白质不易进入树脂孔，因此比较小蛋白质更快地通过柱子。最终，这些蛋白质分子按分子量递减的顺序从柱中洗脱。
Application
凝胶过滤标记物试剂盒（蛋白分子量29,000-700,000 Da）用于：
- 蛋白纯化和分析（凝胶过滤实验）
- 突触神经小体提取物的体积排阻色谱/凝胶过滤色谱
- 甘油梯度离心
- 研究糖皮质诱导淋巴细胞溶解，作为免疫系统中细胞凋亡的模式体系
- 分离玉竹凝集素，其对Vero细胞具有显著的抗HSV-II活性，对A375人黑素瘤细胞具有细胞毒性，并可以半胱天冬酶依赖性方式诱导细胞凋亡
Biochem/physiol Actions
手册所述凝胶过滤色谱法测定分子量是现有发表方法的改良版。该试剂盒中的蛋白标准品可能适合其他色谱方法，如高效液相色谱(HPLC)。但是，某些缓冲体系可能会影响白蛋白和碳酸酐酶的洗脱体积。该试剂盒中的蛋白分子量范围为29 kDa至699 kDa。
Other Notes
- 仅供研究使用。不可用于药物、家庭或其他用途。
- 有关危险和安全处理方法的信息，请参阅安全数据说明书。
- 有关试剂、保存和稳定性、实验方法的信息，请参阅产品信息说明书(product information sheet)。
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- A8531Albumin, bovine serum, 66,000 MW 1 mL/vial
- A8656Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vial
- A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vial
- A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vial
- D4772Blue dextran, 2,000,000 MW 1 mL/vial
- C7025Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vial
- T9145Thyroglobulin, bovine, 669,000 MW 1 mL/vial
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Germán L Rosano et al.
The Journal of biological chemistry, 286(34), 29671-29680 (2011-07-09)
HSP100 proteins are molecular chaperones involved in protein quality control. They assist in protein (un)folding, prevent aggregation, and are thought to participate in precursor translocation across membranes. Caseinolytic proteins ClpC and ClpD from plant chloroplasts belong to the HSP100 family.
D K Wimsatt et al.
Clinical chemistry, 33(11), 2100-2106 (1987-11-01)
The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by
Lei Yue et al.
Nucleic acids research, 48(17), 9589-9605 (2020-08-29)
Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination factor of Archaea
Qi Zong Lao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(12), 5013-5023 (2010-08-25)
Voltage-gated calcium channels conduct Ca(2+) ions in response to membrane depolarization. The resulting transient increase in cytoplasmic free calcium concentration is a critical trigger for the initiation of such vital responses as muscle contraction and transcription. L-type Ca(v)1.2 calcium channels
Gel-filtration chromatography
F'ag'ain 'O?C, et al.
Journal of biological and chemical chronicles, 25-33 (2011)
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