N0163
Nitrate Reductase from Arabidopsis thaliana
vial of ≥0.5 unit
别名:
NADH:nitrate oxidoreductase
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关于此项目
Recombinant:
expressed in Pichia pastoris
recombinant
expressed in Pichia pastoris
Quality Level
form
lyophilized powder
packaging
vial of ≥0.5 unit
shipped in
wet ice
storage temp.
−20°C
Application
Nitrate reductase from Arabidopsis thaliana has been used in a study to assess the amino acid sequence of chicken hepatic sulfite oxidase.
Catalyzes the NADH-dependent reduction of nitrate to nitrite.
Biochem/physiol Actions
Nitrate reductase activity is induced in Arabidopsis thaliana plants by sumoylation via the E3 ligase activity of AtSIZ1.
Physical form
Supplied as a lyophilized powder containing 50 mM MOPS, pH 7.0, 0.1 mM EDTA and a proprietary sugar
Other Notes
One unit will reduce 1.0 micromole of nitrate to nitrite per min in a NADH system at pH 7.5 at 30 deg C.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
Conserved domains in molybdenum hydroxylases. The amino acid sequence of chicken hepatic sulfite oxidase
Neame, P. and M. Barber
The Journal of Biological Chemistry, 164, 20894-20901 (1989)
Arabidopsis nitrate reductase activity is stimulated by the E3 SUMO ligase AtSIZ1
Park, BS
Nature Communications, 19, 400-400 (2011)
Eva Biegel et al.
Environmental microbiology, 11(6), 1438-1443 (2009-02-19)
Acetogenic bacteria grow by the oxidation of various substrates coupled to the reduction of carbon dioxide (acetogenesis) or other electron acceptors but the mechanisms of energy conservation are still enigmatic. Here, we report the presence of a rnf gene cluster
A Kandlbinder et al.
Journal of experimental botany, 51(347), 1099-1105 (2000-08-19)
The activity of nitrate reductase (+Mg(2+), NR(act)) in illuminated leaves from spinach, barley and pea was 50-80% of the maximum activity (+EDTA, NR(max)). However, NR from leaves of Ricinus communis L. had a 10-fold lower NR(act), while NR(max) was similar
Vittoria Martino di Rigano et al.
Plant, cell & environment, 29(7), 1400-1409 (2006-11-04)
Temperature responses of nitrate reductase (NR) were studied in the psychrophilic unicellular alga, Koliella antarctica, and in the mesophilic species, Chlorella sorokiniana. Enzymes from both species were purified to near homogeneity by Blue Sepharose (Pharmacia, Uppsala, Sweden) affinity chromatography and
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N0163-1VL | 04061834092742 |
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