N0252
对硝基苯酚癸
lipase substrate, ≥98% (TLC), powder
别名:
4-硝基苯基癸酸酯, 癸酸4-硝基苯酯
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关于此项目
经验公式（希尔记法）:
C16H23NO4
化学文摘社编号:
分子量:
293.36
Beilstein:
1915230
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
产品名称
对硝基苯酚癸, lipase substrate
质量水平
方案
≥98% (TLC)
表单
powder
溶解性
chloroform: soluble 100 mg/mL, colorless to faintly yellow
储存温度
−20°C
SMILES字符串
CCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C16H23NO4/c1-2-3-4-5-6-7-8-9-16(18)21-15-12-10-14(11-13-15)17(19)20/h10-13H,2-9H2,1H3
InChI key
RRNKCSIEGPOIKI-UHFFFAOYSA-N
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应用
癸酸 4-硝基苯酯可作为底物，以评估重组灰霉青霉 T55 菌株产生的羧酸酯酶。它也已用于肝胰腺提取物的一般酯酶活性测定。
癸酸 4-硝基苯酯已用作分光光度法评估脂肪酶活性的底物。它也已被用作估计羧酸酯酶活性的底物。
生化/生理作用
癸酸 4-硝基苯酯是脂肪酶的底物。与其他 4-硝基苯酯相比，胞外脂肪酶与 4-硝基苯基癸酸酯的水解反应速度更高。
V M Gumerov et al.
Prikladnaia biokhimiia i mikrobiologiia, 48(4), 376-382 (2012-10-06)
As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally
Methods in Molecular biology: Platelets and Megakaryocytes, volume 1, functional assays, 27-27 (2012)
Isolation and characterization of an enzyme from the Greenshell? mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid
MacKenzie LA, et al.
Toxicon, 60(3), 406-419 (2012)
Kamila Dmochowska et al.
Journal of insect science (Online), 13, 77-77 (2013-11-14)
Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae)
Janaina Marques de Almeida et al.
Scientific reports, 8(1), 10000-10000 (2018-07-04)
We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus
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