N0513
神经生长因子-7S 来源于鼠颔下腺
lyophilized powder, suitable for cell culture
别名:
NGF-7S
产品名称
神经生长因子-7S 来源于鼠颔下腺, NGF-7S, lyophilized powder, suitable for cell culture
生物来源
mouse
质量水平
表单
lyophilized powder
效能
2-250 ng/mL ED50/EC50
质量
endotoxin tested
分子量
protein 130 kDa
包装
pkg of 0.1 mg
技术
cell culture | mammalian: suitable
杂质
≤10
颜色
white
UniProt登记号
储存温度
−20°C
基因信息
mouse ... Ngf(18049)
一般描述
神经生长因子 (NGF) 是由 118 个残基的多肽链非共价连接而成的二聚体。这两种多肽都含有三个分子内二硫键。它形成了神经营养因子家族的原型。在雄性小鼠颌下腺中，NGF 的含量较高，是一种高分子量的复合物，称为神经生长因子-7S (NGF-7S)。NGF-7S 是一种 α2β2γ2复合物，其中 β2 NGF 是具有活性的神经营养因子，并与腺激肽释放酶丝氨酸蛋白酶成员 α-NGF 和 γ-NGF 的二聚体连接。
应用
来自小鼠颌下腺的神经生长因子-7S (NGF-7S) 已被用于处理以下细胞以诱导神经元分化：
- PC12
- 背根神经节 (DRG) 获自两种性别的鸡胚（原鸡）
生化/生理作用
神经生长因子 (NGF) 是一种神经营养因子，具有促进中枢神经系统和周围神经系统特定亚型神经元分化和存活的作用。然而，神经生长因子-7S (NGF-7S) 缺乏任何神经营养活性，因为大部分 β——NGF 亚基被埋没在 NGF-7S 复合体中。
外形
0.2μ 冻干25 mM 磷酸钠 (pH 7.0) 中的 m 过滤溶液。
制备说明
神经生长因子-7S (NGF-7S) 是从雄性小鼠颌下腺中分离得到的，使用了Varon 等人的改良后方法。
分析说明
采用 3 天 MTT 试验测定生物活性。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Kun-Shan Huang et al.
Evidence-based complementary and alternative medicine : eCAM, 2011, 109809-109809 (2009-08-19)
The present study provides in vitro and in vivo evaluation of Paeoniae alba Radix (PR) on peripheral nerve regeneration. In the in vitro study, we found the PR caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from
Julien Falk et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(2), 373-391 (2014-01-10)
The elongation rate of axons is tightly regulated during development. Recycling of the plasma membrane is known to regulate axon extension; however, the specific molecules involved in recycling within the growth cone have not been fully characterized. Here, we investigated
Hsien-Tung Chen et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 84(1), 256-262 (2007-05-22)
This study provides in vitro and in vivo evaluation of rat serum metabolites of the Pueraria lobata (SMP) on peripheral nerve regeneration. In the in vitro study, we found that the SMP caused a marked enhancement of the nerve growth
Phong Duong et al.
Biology of sex differences, 11(1), 12-12 (2020-04-01)
The role of sex hormones on cellular function is unclear. Studies show androgens and estrogens are protective in the CNS, whereas other studies found no effects or damaging effects. Furthermore, sex differences have been observed in multiple oxidative stress-associated CNS
Héctor I Rocha-González et al.
Journal of neurophysiology, 100(1), 169-184 (2008-04-04)
Adult primary afferent neurons are depolarized by GABA throughout their entire surface, including their somata located in dorsal root ganglia (DRG). Primary afferent depolarization (PAD) mediated by GABA released from spinal interneurons determines presynaptic inhibition, a key mechanism in somatosensory
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