General description
Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.
Application
Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.
Other Notes
0.5 N NaOH, 1.5 M NaCl
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.
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Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
存储类别
8B - Non-combustible corrosive hazardous materials
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable