N1665
5′-核苷酸酶 人
recombinant, expressed in CHO cells, vial of 6-12 μg
别名:
CD73, NT5E, 外-5′-核苷酸酶
重组
expressed in CHO cells
质量水平
描述
contains a C-terminal 6-His tag
方案
≥90% (SDS-PAGE)
表单
solution
enzyme activity
≥15 units/mg protein
分子量
~61 kDa by SDS-PAGE (reducing)
包装
vial of 6-12 μg
杂质
≤1.0 EU/μg endotoxin
NCBI登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... NT5E(4907)
一般描述
5′-核苷酸酶是广泛存在于哺乳动物细胞中的胞外酶。有助于从 5′ 加速磷酸基的水解反应核苷酸，产生相应的核苷。
生化/生理作用
5′-核苷酸酶 (nucleotidase) 又称 CD73，是一种能转化核苷-5′−单磷酸生成核苷，底物偏好 AMP。天然 5'-核苷酸酶是一种 GPI 锚定蛋白，其暴露量因缺氧而上调。5′-核苷酸酶在体内有许多功能 ，包括产生细胞外腺苷。
5′-核苷酸酶具有多种临床意义。在体外肿瘤血管生成和体内肿瘤免疫逃逸中，它是调控肿瘤细胞增殖、迁移和侵袭的关键分子。由于这一重要角色，该酶是癌症研究的潜在靶点。也参与细胞外核苷酸的补救，并在组织稳态的控制中起关键作用。
外形
提供含 Tris、NaCl、CaCl 2、 和 20% 甘油的溶液。
其他说明
一个单元将-37℃、pH 9.0 时每分钟从单磷酸盐水解 1.0μmol腺苷 5′-无机磷。
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Isolation of a plasma-membrane subfraction from rat liver containing an insulin-sensitive cyclic-AMP phosphodiesterase.
P D House et al.
European journal of biochemistry, 24(3), 429-437 (1972-01-21)
Zhao-wei Gao et al.
BioMed research international, 2014, 460654-460654 (2014-08-16)
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of
Anna Wilk et al.
Scientific reports, 10(1), 651-651 (2020-01-22)
Changes in nicotinamide adenine dinucleotide (NAD+) levels that compromise mitochondrial function trigger release of DNA damaging reactive oxygen species. NAD+ levels also affect DNA repair capacity as NAD+ is a substrate for PARP-enzymes (mono/poly-ADP-ribosylation) and sirtuins (deacetylation). The ecto-5'-nucleotidase CD73
Paul A Beavis et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(36), 14711-14716 (2013-08-22)
CD73 inhibits antitumor immunity through the activation of adenosine receptors expressed on multiple immune subsets. CD73 also enhances tumor metastasis, although the nature of the immune subsets and adenosine receptor subtypes involved in this process are largely unknown. In this
5'-Nucleotidase: molecular structure and functional aspects.
H Zimmermann
The Biochemical journal, 285 ( Pt 2), 345-365 (1992-07-15)
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